| 黄燕,单丽,邱水生,等.益肾降糖胶囊质量控制方法的研究[J].安徽医药,2021,25(4):673-677. |
| 益肾降糖胶囊质量控制方法的研究 |
| Study on the quality control method of Yishen Jiangtang capsules |
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| DOI：10.3969/j.issn.1009-6469.2021.04.009 |
| 中文关键词: 中草药 主成分分析 色谱法，薄层 色谱法，高压液相 益肾降糖胶囊 质量控制 芍药苷 二苯乙烯苷 黄芩苷 |
| 英文关键词: Drugs, Chinese Herbal Principal component analysis Chromatography, thin layer Chromatography, high pressure liquid Yishen Jiangtang capsule Quality control Paeoniflorin Stilbene glucoside Baicalin |
| 基金项目:福建省科技厅引导性项目(2018Y0044) |
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| 中文摘要: |
| 目的建立益肾降糖胶囊的质量控制方法。方法 2018年9月至 2019年3月，运用薄层色谱法(TLC)对益肾降糖胶囊中的制何首乌、赤芍、玄参、黄芪、山药、黄芩进行定性鉴别；采用高效液相色谱法测定芍药苷、二苯乙烯苷和黄芩苷的含量。采用 Ultimate? XB-C18(4.6×250 mm，5 μm)色谱柱；以甲醇 -0.2%磷酸溶液为流动相，梯度洗脱；流速为 1.0 mL/min；检测波长为 230 nm(芍药苷)和 320 nm(二苯乙烯苷、黄芩苷)柱温为 40 ℃。结果制何首乌、赤芍、玄参等 TLC图斑点清晰，分离度好，阴性对照无干扰。芍药苷、二苯乙烯苷、黄芩苷质量浓，度分别在 31.320～ 234.900 μg/mL(r=0.999 1)3.939～14.772 μg/mL(r= |
| 英文摘要: |
| Objective To establish the quality control method of Yishen Jiangtang capsules.Methods From September 2018 toMarch 2019, thin layer chromatography (TLC) was used for the qualitative identification of Polygoni multiflori radix praeparata, Paeoniae radix rubra, Scrophulariae radix, Astragali radix, Dioscoreae rhizoma and Scutellariae radix in Yishen Jiangtang capsules. HPLCmethod was adopted for the contents determination of paeoniflorin, Stilbene glucoside and Baicalin. The analysis was carried out on anUltimate? XB-C18 column (4.6×250 mm, 5 μm). The mobile phase was composed of methanol –0.2%phosphoric acid aqueous withgradient elution at the flow rate of 1.0 mL/min. The detection wavelength was set at 230 nm (paeoniflorin) and 320 nm (stilbene glucoside, baicalin), the column temperature was 40 ℃.Results The TLC sports of Polygoni multiflori radix praeparata, Paeoniae radix rubra, Scrophulariae radix and Astragali radix were clear and well separated without the interference of negative samples. Paeoniflorin,Stilbene glucoside and Baicalin had good linear relationships within the ranges of 31.320-234.900 μg/mL (r=0.999 1), 3.939-14.772 μg/mL (r=0.999 5), 36.080-270.600 μg/mL (r=0.999 5), whose average recoveries were 98.96%, 99.91%, 99.89% with the RSDs of 0.76%, 1.16% and 1.41% (n=6), respectively.Conclusion The method is simple and accurate with good repeatability, it can be used to control the quality of Yishen Jiangtang capsules. |
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