| 丁彩云,王梦瑾,孙立清.不同咽拭子取样肺炎支原体 DNA检测对支原体肺炎诊断价值研究[J].安徽医药,2021,25(4):733-736. |
| 不同咽拭子取样肺炎支原体 DNA检测对支原体肺炎诊断价值研究 |
| Study on the diagnostic value of mycoplasma pneumoniae DNA detection from different throat swabs in children with mycoplasma pneumoniae infection |
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| DOI：10.3969/j.issn.1009-6469.2021.04.024 |
| 中文关键词: 肺炎，支原体 痰 咽拭子取样 肺炎支原体 DNA检测 乙酰半胱氨酸 免疫球蛋白 M 诊断价值 |
| 英文关键词: Pneumonia, mycoplasma Sputum Throat swab sampling Mycoplasma pneumoniae DNA detection Acetylcysteine Immunoglobulin M Diagnostic value |
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| 中文摘要: |
| 目的探讨不同咽拭子取样肺炎支原体 DNA检测对支原体感染性肺炎病儿诊断价值。方法选择 2017年 2月至 2019年 6月在蚌埠市第一人民医院接受治疗的疑似肺炎支原体感染住院病儿 174例进行研究。所有病儿入院后均由同一组护士采用常规咽拭子取样与改良咽拭子取样两种咽拭子采集方式对气道分泌物进行采集并行支原体 DNA检测。参照诸福棠《实用儿科学》第 7版中的诊断标准为金标准，分析两种咽拭子取样方式肺炎支原体 DNA检测对支原体感染性肺炎病儿的诊断效能。结果 174例病儿中 37例(21.26%)经金标准诊断确诊为支原体感染性肺炎。常规咽拭子取样肺炎支原体 DNA检测与金标准诊断结果一致性尚可(Kappa=0.526，P<0.001)常规咽拭子取样肺炎支原体 DNA检测对支原体感染性肺炎诊断的灵敏度、特异度、确度、阳性预测值、阴性预测值分别为 78.38，%(29/37)、 82.48%(113/137)、 81.61%(142/174)、 54.72%(29/53)、 93.39%(113/121)。准改良咽拭子取样肺炎支原体 DNA检测与金标准诊断结果一致性良好(Kappa=0.781，P<0.001)改良咽拭子取样肺炎支原体 DNA检测对支原体感染性肺炎诊断的灵敏度、特异度、准确度、阳性预测值、阴性预测值分别为 94.，59%(35/37)、 91.24%(125/137)、 |
| 英文摘要: |
| Objective To explore the diagnostic value of Mycoplasma pneumoniae DNA detection from different throat swabs inchildren with Mycoplasma pneumoniae infection.Methods One hundred and seventy-four hospitalized children with suspected Mycoplasma pneumoniae infection who were treated at Bengbu First People's Hospital from February 2017 to June 2019 were selected forthe study. All patients were admitted to the hospital by the same group of nurses using conventional nasal sampling and modified pharyngeal swab sampling to collect airway secretions and perform mycoplasma DNA testing. According to the gold standard in the 7th edition of Zhu Fuzhen's Practical Pediatrics, the diagnostic efficacy of two kinds of throat swab sampling methods for Mycoplasma pneumoniae DNA detection in children with mycoplasma-infected pneumonia was analyzed.Results Of the 174 patients, 37 (21.26%) werediagnosed as mycoplasma pneumonia by gold standard diagnosis. Conventional throat swab sampling was consistent with gold standarddiagnostic results (Kappa=0.526, P<0.001). The sensitivity, specificity and accuracy of conventional throat swab sampling for detectionof mycoplasma pneumonia degree, positive predictive value, and negative predictive value were 78.38% (29/37), 82.48% (113/137),81.61% (142/174), 54.72% (29/53), and 93.39% (113/121). The improved throat swab samples were consistent with the gold standarddiagnosis (Kappa=0.781, P<0.001). The sensitivity, specificity and accuracy of the improved throat swab sampling for detection of mycoplasma pneumonia were detected. The positive predictive value and negative predictive value were 94.59% (35/37), 91.24% (125/137), 91.95% (160/174), 74.47% (35/47), and 98.43% (125/127). The sensitivity, specificity, accuracy and positive predictive value ofthe diagnostic efficiency of the original swine pneumonia were significantly higher than those of the conventional throat swab (P<0.05). Conclusions The throat swab sampling DNA detection of Mycoplasma pneumoniae has a high diagnostic value for children with my‐coplasma-infected pneumonia. The improved throat swab sampling can effectively improve the diagnostic sensitivity, specificity, accuracy and positive predictive value. |
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