| 李刚,刘明,王太鹏.唑来膦酸对骨肉瘤细胞增殖、迁移和侵袭的影响及其作用机制[J].安徽医药,2021,25(9):1723-1727. |
| 唑来膦酸对骨肉瘤细胞增殖、迁移和侵袭的影响及其作用机制 |
| Effects of Zoledronate on proliferation, migration and invasion of osteosarcoma cells and the mechanism |
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| DOI：10.3969/j.issn.1009-6469.2021.09.006 |
| 中文关键词: 骨肉瘤 唑来膦酸 微小 RNA-520a-3p T细胞特异性转录因子 7 增殖 迁移 侵袭 |
| 英文关键词: Osteosarcoma Zoledronate miR-520a-3p TCF7 Proliferation Migration Invasion |
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| 中文摘要: |
| 目的研究唑来膦酸对骨肉瘤细胞增殖、迁移和侵袭的影响，并探讨其作用机制。方法将对照组(未做任何处理)、唑来膦酸( ZOL)组( 50 μmol/L唑来膦酸处理)、 miR-con组(转染 miR-con)、 miR-520a-3p组(转染 miR-520a-3p mimics)、 ZOL+anti-miR-con组(转染 anti-miR-con再用唑来膦酸处理)、 ZOL+anti-miR-520a-3p组(转染 anti-miR-520a-3p再用唑来膦酸处理)，均用脂质体法转染至人骨肉瘤细胞( HOS)； MTT法检测各组细胞的增殖； Transwell法检测各组细胞的迁移、侵袭；实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测各组细胞中 miR-520a-3p的表达；蛋白质印迹法( Western blotting)检测各组细胞中 T细胞特异性转录因子 7(TCF7)的蛋白表达。双荧光素酶报告基因检测实验检测细胞的荧光活性。结果与对照组相比， ZOL组 HOS细胞的吸光度［(1.05±0.11)比( 0.73±0.07)］迁移细胞数［(86.49±9.17)个比( 31.67±4.29)个］、侵袭细胞数［(64.17±7.72)个比( 17.82±3.19)个］显著降低， miR-520a-3p的表达、明显下调( P<0.05)；过表达 miR-520a-3p可升高 HOS细胞的吸光度［( 1.09±0.12)比( 0.68±0.09)］、迁移细胞数［( 88.49±9.17)个比( 35.76±5.14)个］、侵袭细胞数［( 64.17±7.72)个比( 19.48±4.27)个］； TCF7是 miR-520a-3p的靶标。抑制 miR-520a-3p可逆转 ZOL对 HOS细胞增殖、迁移、侵袭的抑制作用，且可部分逆转 ZOL对 HOS细胞中 TCF7表达的抑制作用。结论唑来膦酸可能通过调控 miR-520a-3p/TCF7轴抑制骨肉瘤 HOS细胞的增殖、迁移和侵袭。 |
| 英文摘要: |
| Objective To study the effects of Zoledronate on proliferation, migration and invasion of osteosarcoma cells, and to explore its mechanism.Methods Control group (without any treatment), ZOL group (50 μmol/L Zoledronate treatment), miR-con group (transfected miR-con), miR-520a-3p group (transfected miR-520a-3p mimics), ZOL+anti-miR-con group (transfected anti-miR-con and then treated with Zoledronate), ZOL+anti-miR-520a-3p group (transfected anti-miR-520a-3p and then treated with Zoledronate) weretransfected into human osteosarcoma (HOS) cells by liposome method. The proliferation of cells in each group was detected by methylthiazolyl tetrazolium (MTT) assay, the migration and invasion of cells in each group were detected by Transwell method, the expressionof miR-520a-3p was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and the protein expression of T cell-specific transcription factor 7 (TCF7) in each group was detected by Western blotting. Double luciferase reporter gene assay was used to detect the fluorescence activity of cells.Results Compared with the control group, the absorption [(1.05±0.11) vs. (0.73±0.07)], migration [(86.49±9.17) vs. (31.67±4.29)] and invasion [(64.17±7.72) vs. (17.82±3.19)] number of HOS cells in the ZOL group were significantly decreased, and the expression of miR-520a-3p was significantly down-regulated (P <0.05). Overexpression of miR-520a-3p increased the absorption [(1.09±0.12) vs. (0.68±0.09)], the number of migration [(88.49±9.17) vs. (35.76±5.14)] and invasion [(64.17±7.72) vs. (19.48±4.27)] of HOS cells. TCF7 was the target of miR-520a-3p. Inhibition of miR-520a-3p reversed the inhibitory effect of ZOL onproliferation, migration and invasion of HOS cells, and partially reversed the inhibitory effect of ZOL on TCF7 expression in HOS cells.Conclusion Zoledronate may inhibit the proliferation, migration and invasion of HOS cells by regulating the miR-520a-3p/TCF7 axis. |
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