| 秦瑞,施晓星.白花蛇舌草总黄酮通过下调 NOX4表达对前列腺癌细胞侵袭、迁移、 EMT的影响[J].安徽医药,2021,25(10):1929-1933. |
| 白花蛇舌草总黄酮通过下调 NOX4表达对前列腺癌细胞侵袭、迁移、 EMT的影响 |
| Effects of total flavonoids from hedyotis diffusa on invasion, migration and EMT of prostate cancer cells by down regulating the expression of NOX4 |
| |
| DOI：10.3969/j.issn.1009-6469.2021.10.005 |
| 中文关键词: 白花蛇舌草 黄酮类 前列腺癌 pc-3细胞 尼克酰胺腺嘌呤二核苷酸磷酸氧化酶 4 细胞侵袭转移 上皮间质转化 |
| 英文关键词: Oldenlandia Flavones Pc-3 NOX4 Cell invasion and metastasis EMT |
| 基金项目: |
|
| 摘要点击次数: 1233 |
| 全文下载次数: 402 |
| 中文摘要: |
| 目的研究白花蛇舌草总黄酮对前列腺癌 pc-3细胞侵袭和转移的影响并探讨其分子机制。方法本研究于 2019年 3 —10月在枣庄矿业集团中心医院实验室进行。分别以 0.1 g/L、0.25 g/L、0.5 g/L浓度的白花蛇舌草总黄酮， 10 μmol/L尼克酰胺腺嘌呤二核苷酸磷酸氧化酶 4(NOX4)的抑制剂氯化二亚苯基碘鎓( DPI)(阳性对照)作用于体外培养的前列腺癌 pc-3细胞，细胞计数试剂( CCK-8)法分别在 24 h、48 h后检测 pc-3细胞增殖情况，筛选合适药物作用时间；以 Transwell侵袭实验和细胞划痕实验检测白花蛇舌草总黄酮对 pc-3细胞侵袭迁移能力的影响；采用免疫印记( WB)法检测上皮间质转化( EMT)标志蛋白钙黏蛋白 E(E-cadherin)、波形纤维蛋白( Vimentin)和 NOX4及两面神激酶 2(JAK2)/信号转导及转录活化因子 3(STAT3)信号通路相关蛋白的表达。结果与空白对照组相比，白花蛇舌草总黄酮和 DPI都能在 24h、48h抑制其细胞活力( 24h:(92.6±1.62)%比(100.0±1.31)%、(76.9±1.54)%比( 100.0±1.31)%、(63.9±1.64)%比( 100.0±1.31)%、(61.3±1.44)%比( 100.0±1.31)%)，差异有统计学意义( P<0.05)，且白花蛇舌草总黄酮各浓度之间有浓度依赖性，白花蛇舌草总黄酮高浓度组与 DPI组相比，差异无统计学意义( P>0.05)根据 CCK-8实验结果选择药物作用 pc-3细胞 16h继续后续实验；与空白对照组相比，白花蛇舌草总黄酮各浓度和 DPI处理 pc细胞后， pc-3细胞侵袭迁移能力［侵袭( 120.32±18.51)个比( 174.81±20.13)个、(71.60±10.12)个比( 174.81± -3，20.13)个、(32.08±5.17)个比( 174.81±20.13)个、(38.13±8.32)个比( 174.81±20.13)个；迁移( 830.24±80.16)个比( 1241.78± 120.12)个、(510.23±61.34)个比( 1241.78±120.12)个、(280.29±30.18)个比( 1241.78±120.12)个、(298.91±40.31)个比( 1241.78± 120.12)个］降低， E-cadherin蛋白表达［( 0.83±0.18)比( 0.41±0.12)、(1.26±0.16)比( 0.41±0.12)、(2.08±0.19)比( 0.41±0.12)、(2.11±0.13)比( 0.41±0.12)］升高， Vimentin、NOX4蛋白表达［( 0.90±0.18)比( 1.21±0.11)、(0.46±0.13)比( 1.21±0.11)、(0.28±0.20)比( 1.21±0.11)、(0.21±0.23)比( 1.21±0.11)］和 JAK2/STAT3信号通路相关蛋白 p-JAK2/JAK2、p-STAT3/STAT3降低，差异有统计学意义(P<0.05)，且白花蛇舌草总黄酮各浓度之间有浓度依赖性，白花蛇舌草总黄酮高浓度组与 DPI组相比，差异无统计学意义(P>0.05)。结论白花蛇舌草总黄酮能降低前列腺癌 pc-3细胞侵袭转移能力，可能是通过下调 NOX4表达实现的。 |
| 英文摘要: |
| Objective To study the effects of total flavonoids from Hedyotis diffusa on invasion and metastasis of prostate cancer PC3 cells and explore its molecular mechanism.Methods This study was conducted in the laboratory of Zaozhuang Mining Group Central Hospital from March 2019 to October 2019. Prostate cancer PC-3 cells cultured in vitro were treated with total flavonoids from Hedyotis diffusa at concentrations of 0.25, 0.5 and 1 g/L and 10 μmol/L nicotinamide adenine dinucleotide phosphate oxidase 4 inhibitor Phenyliodonium (DPI) (positive control) respectively, the proliferation of PC-3 cells was detected by cell counting reagent CKK-8 method after 24h and 48h respectively, screened the appropriate time for drug action. Transwell invasion test and cell scratch test wereused to detect the effects of total flavonoids from Hedyotis diffusa on the invasion and migration of PC-3 cells; the expressions of epithelial-mesenchymal transition (EMT) marker proteins E-cadherin, Vimentin, NOX4 and Janus kinase 2 (JAK2)/signal transducers and activators of transcnption 3 (STAT3) signaling pathway related protein were detected by immunoblotting (WB).Results Compared withcontrol group, total flavonoids from Hedyotis diffusa and DPI could inhibit the cell viability at 24 h and 48 h [24 h: (92.6±1.62)% vs. (100.0±1.31)%, (76.9±1.54)% vs. (100.0±1.31)%; 48 h: (63.9±1.64)% vs. (100.0±1.31)%, (61.3±1.44)% vs. (100.0±1.31)%], the differences were significant (P<0.05), there was a concentration dependence between the concentrations of total flavonoids from Hedyotis dif fusa, there was no significant difference between the high concentration total flavonoids from Hedyotis diffusa group and DPI group (P> 0.05); according to CCK-8 test results, drug-treated PC-3 cells for 16 h were selected to continue the follow-up experiment; compared withcontrol group, after treated with different concentrations total flavonoids from Hedyotis diffusa and DPI, the invasion and migration abilities of PC-3 cells decreased [invasion: (120.32±18.51) vs. (174.81±20.13), (71.60±10.12) vs. (174.81±20.13), (32.08±5.17) vs. (174.81± 20.13), (38.13±8.32) vs. (174.81±20.13); migration: (830.24±80.16) vs. (1241.78±120.12), (510.23±61.34) vs. (1241.78±120.12), (280.29±30.18) vs. (1241.78±120.12), (298.91±40.31) vs. (1241.78±120.12)], the expression of E-cadherin protein increased [(0.83± 0.18) vs. (0.41±0.12), (1.26±0.16) vs. (0.41±0.12), (2.08±0.19) vs. (0.41±0.12), (2.11±0.13) vs. (0.41±0.12)], the expressions of Vimentin, NOX4 protein [(0.90±0.18) vs. (1.21±0.11), (0.46±0.13) vs. (1.21±0.11), (0.28±0.20) vs. (1.21±0.11), (0.21±0.23) vs. (1.21±0.11)] and JAK2/STAT3 signaling pathway related proteins p-JAK2/JAK2, p-STAT3/STAT3 decreased, the differences were significant (P<0.05),there was a concentration dependence between the concentrations of total flavonoids from Hedyotis diffusa, there was no significant difference between the high concentration group and DPI group (P>0.05).Conclusion Total flavonoids from Hedyotis diffusa can reduce the invasive and metastatic abilities of prostate cancer PC-3 cells, probably be achieved by down-regulating the expression of NOX4. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
