| 陆智芸,许水森,农应全.重组人干扰素 γ对变应性鼻炎大鼠 Toll样受体 4/核因子 -B信号通路及血清炎症因子的影响[J].安徽医药,2021,25(11):2126-2130. |
| 重组人干扰素 γ对变应性鼻炎大鼠 Toll样受体 4/核因子 -B信号通路及血清炎症因子的影响 |
| Effect of recombinant human interferon gamma on TLR4/NF-B signaling pathway and serum inflammatory factors in allergic rhinitis rats |
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| DOI：10.3969/j.issn.1009-6469.2021.11.002 |
| 中文关键词: 鼻炎，变应性 重组人干扰素 γ 炎症因子 TLR4/NF-B信号通路 |
| 英文关键词: Rhinitis,allergic Recombinant human interferon gamma Inflammatory factor TLR4 / NF-B signal pathway |
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| 中文摘要: |
| 目的分析重组人干扰素 γ对变应性鼻炎大鼠 Toll样受体( TLR4)/核因子 -B(NF-B)信号通路及血清炎症因子影响。方法选取 SPF级 Wistar雄性大鼠 60只，随机数字表法将大鼠分为正常组、模型组与实验组，模型组与实验组制备 AR模型，由第 22天建立模型后，依据人和动物剂量转换公式，实验组大鼠采用 20 IU的重组人干扰素 γ(IFN-γ)滴鼻治疗， 1次/天，正常组与模型组采用等剂量生理盐水滴鼻，持续 14 d。观察各组大鼠行为学评分、鼻黏膜病理形态、血清炎症因子含量及鼻黏膜组织 TLR4、NF-B p65蛋白与 mRNA表达情况。结果强化致敏 7d完成后，实验组大鼠行为学评分为( 7.68±1.52)分，模型组为(7.81±1.43)分，均高于正常组的( 2.95±0.57)分，差异有统计学意义( t=13.031、14.119，P=0.001、0.001)；末次给药后实验组大鼠流涕、喷嚏和挠鼻等状况较模型组显著好转，行为学评分降低至( 3.98±0.64)分，较模型组的( 8.49±1.53)分差异有统计学意义(t =12.161，P=0.001)。模型组大鼠鼻黏膜内肥大细胞、嗜酸粒细胞数量较正常组升高，实验组大鼠鼻黏膜内肥大细胞、嗜酸粒细胞数量较模型组降低，差异有统计学意义( P<0.05)。模型组大鼠血清 IFN-γ、白细胞介素 -10(IL-10)含量低于正常组，免疫球蛋白 E(IgE)、肿瘤坏死因子 -α(TNF-α)、白细胞介素 -4(IL-4)、白细胞介素 -6(IL-6)含量高于正常组；实验组大鼠血清 IFN-γ、IL-10含量高于模型组， IgE、TNF-α、IL-4、IL-6含量低于模型组，差异有统计学意义( P<0.05)。模型组大鼠鼻黏膜组织 TLR4、NF-B p65蛋白与 mRNA表达较正常组升高，实验组大鼠鼻黏膜组织 TLR4、NF-B p65蛋白与 mRNA表达较模型组降低，差异有统计学意义(P<0.05)。结论重组人干扰素 γ可降低变应性鼻炎大鼠血清炎症因子含量，减少生成 IgE，调节 T细胞免疫失衡，其作用机制可能和抑制 TLR4/NF-B信号通路表达有关。 |
| 英文摘要: |
| Objective To analyze the effect of recombinant human interferon γ on TLR4 / NF-B signaling pathway and serum inflammatory factors in allergic rhinitis (AR) rats.Methods Sixty SPF Wistar male rats were selected and divided into normal group,model group and experimental group by random number table method. AR models were prepared in the model group and experimentalgroup. On the 22nd day after model establishment, according to the human and animal dose conversion formula, rats in the experimental group were treated with 20 IU of recombinant human IFN-γ nasal drops intranasally once a day, and the normal group and the modelgroup received nasal drops of normal saline for 14 days. The behavioral scores, pathological morphology of nasal mucosa, the content ofserum inflammatory factors and the expression of TLR4, NF-B p65 protein and mRNA in nasal mucosa of each group of rats were observed.Results After 7 days of intensive sensitization, the behavioral score of rats was (7.68±1.52) points in the experimental groupand (7.81±1.43) points in the model group, which were higher than that in the the normal group (2.95±0.57) points. The differenceswere statistically significant (t=13.031, 14.119, P=0.001, 0.001); after the last administration, the conditions of runny nose, sneezing,and scratching the nose in the experimental group were significantly improved compared with the model group, and the behavioral scorewas reduced to (3.98±0.64) points. Compared with the model group (8.49±1.53), the difference was statistically significant (t=12.161, P =0.001). The number of mast cells and eosinophils in the nasal mucosa of the model group rats was higher than that in the normalgroup, while the number of mast cells and eosinophils in the nasal mucosa of the experimental group rats was lower than that in themodel group. The difference was statistically significant (P<0.05). The levels of serum IFN-γ and IL-10 in the model group were lower than those in the normal group, and the contents of IgE, TNF-α, IL-4, and IL-6 were higher than those in the normal group; Compared with the model group, the contents of IgE, TNF-α, IL-4 and IL-6 were lower than the model group, and the difference was statistically significant (P<0.05). The expressions of TLR4, NF-B p65 protein and mRNA in the nasal mucosa of the model group rats were higherthan those in the normal group, while the expressions of TLR4, NF-B p65 protein and mRNA in the nasal mucosa of the model grouprats were lower than those in the model group, and the differences were statistically significant (P<0.05).Conclusion Recombinant human interferon γ can reduce serum inflammatory factor content, reduce IgE production, and regulate T cell immune imbalance in ratswith allergic rhinitis. The mechanism may be related to the inhibition of TLR4 / NF-B signaling pathway expression. |
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