| 向从明,陈友干,孙承文,等.微小 RNA-15a-5p靶向蛋白质二硫键异构酶 A6前体蛋白抑制前列腺癌细胞增殖、迁移及侵袭[J].安徽医药,2021,25(11):2214-2218. |
| 微小 RNA-15a-5p靶向蛋白质二硫键异构酶 A6前体蛋白抑制前列腺癌细胞增殖、迁移及侵袭 |
| miR-15a-5p inhibits prostate cancer cell proliferation, migration and invasion by targeting PDIA6 |
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| DOI：10.3969/j.issn.1009-6469.2021.11.021 |
| 中文关键词: 前列腺肿瘤 蛋白质二硫化物异构酶类 微小 RNA-15a-5p 蛋白质二硫键异构酶 A6前体蛋白 增殖 迁移 侵袭 |
| 英文关键词: Prostatic neoplasms Protein disulfide-isomerases Migration Invasion |
| 基金项目:中国博士后基金( 2015M571670) |
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| 中文摘要: |
| 目的探讨微小 RNA-15a-5p(miR-15a-5p)是否可通过靶向蛋白质二硫键异构酶 A6前体蛋白( PDIA6)而抑制前列腺癌细胞增殖、迁移及侵袭。方法选取 2018年 2月至 2019年 3月江南大学附属医院接受治疗的前列腺癌病人 50例，采用实时荧光定量 PCR(qRT-PCR)与蛋白印迹法( Western blotting)分别检测前列腺癌组织、癌旁组织中 miR-15a-5p、PDIA6的表达量；体外培养人前列腺癌 DU145细胞，将细胞分为 miR-NC组、 miR-15a-5p组、 si-NC组、 si-PDIA6组、 miR-15a-5p+pcDNA组、 miR15a-5p+pcDNA-PDIA6组；噻唑蓝( MTT)检测细胞增殖； Transwell小室实验检测细胞迁移及侵袭；双荧光素酶报告实验验证 miR-15a-5p、PDIA6的靶向关系。结果与癌旁组织相比，前列腺癌组织中 miR-15a-5p的表达水平降低［(1.00±0.17)比( 0.68±0.11)］，PDIA6 mRNA［( 0.99±0.09)比( 1.64±0.18)］和蛋白［( 0.44±0.07)比( 0.76±0.17)］表达水平升高；转染 miR-15a-5p mimics或转染 si-PDIA6可降低细胞存活率( P<0.05)减少迁移及侵袭细胞数( P<0.05)；双荧光素酶报告实验证实 miR-15a-5p可靶向结合 PDIA6；PDIA6过表达可降低 miR-15a-5p表达对 DU145细胞增殖、迁移及侵袭的抑制作用。结论过表达 miR-15a-5p过，通过降低 PDIA6的表达对列腺癌细胞增殖、迁移及侵袭能力有抑制作用。 |
| 英文摘要: |
| Objective To investigate whether miR-15a-5p could inhibit the proliferation, migration and invasion of prostate cancer cells by targeting PDIA6.Methods qRT-PCR and Western blotting were used to detect the expression of miR-15a-5p and PDIA6 inprostate cancer tissues and adjacent tissues. Human prostate cancer DU145 cells were cultured in vitro and divided into miR-NC group, miR-15a-5p group, si-NC group, si-PDIA6 group, miR-15a-5p+pcDNA group, miR-15a-5p+pcDNA -PDIA6 group. MTT wasused to detect the cell proliferation. The Transwell cell test was used to detect the cell migration and invasion. The dual luciferase report experiment verified the targeting relationship between miR-15a-5p and PDIA6.Results Compared with adjacent tissues, the expression level of miR-15a-5p in prostate cancer tissue decreased [(1.00±0.17) vs. (0.68±0.11)], and the expression level of PDIA6 mRNA [(0.99±0.09) vs. (1.64±0.18)] and protein [(0.44±0.07) vs. (0.76±0.17)] increased. Transfection of miR-15a-5p mimics or transfection of si-PDIA6 could reduce cell survival rate (P<0.05), and reduce the number of migrating and invading cells (P<0.05). The dual luciferase report experiment confirmed that miR-15a-5p could target PDIA6. PDIA6 overexpression could reduce the inhibitory effect of miR-15a-5p overexpression on DU145 cell proliferation, migration and invasion.Conclusion Overexpression of miR-15a-5p inhibites the proliferation, migration and invasion of prostate cancer cells by reducing the expression of pdia6. |
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