| 伊纪亮,郑娟,张萍.LncRNA KCNQ1OT1通过调控 miR-506-3p表达调节喉鳞状细胞癌细胞的生物学行为[J].安徽医药,2022,26(1):151-155. |
| LncRNA KCNQ1OT1通过调控 miR-506-3p表达调节喉鳞状细胞癌细胞的生物学行为 |
| LncRNA KCNQ1OT1 regulates biological behavior of laryngeal squamous cell carcinoma cells by regulating miR-506-3p expression |
| |
| DOI：10.3969/j.issn.1009-6469.2022.01.035 |
| 中文关键词: 喉肿瘤 癌，鳞状细胞 长链非编码 RNA(LncRNA) KCNQ1重叠转录物 1(KCNQ1OT1) miR-506-3p 细胞增殖 凋亡 迁移 侵袭 |
| 英文关键词: Laryngeal neoplasms Carcinoma, squamous cell LncRNA KCNQ1OT1 miR-506-3p Cell proliferation Apoptosis Migration Invasion |
| 基金项目: |
|
| 摘要点击次数: 1091 |
| 全文下载次数: 394 |
| 中文摘要: |
| 目的探讨长链非编码 RNA(LncRNA)KCNQ1重叠转录物 1(KCNQ1OT1)对喉鳞状细胞癌细胞生物学的影响及作用机制。方法于 2019年 5月至 2020年 2月，采用 RT-qPCR法检测了 45例来自山东大学齐鲁医院桓台分院喉鳞状细胞癌病人的癌组织和癌旁组织及购自上海研生实业有限公司的人胚肺成纤维细胞 WI-38和购自中国科学院上海细胞库的喉鳞状细胞癌细胞系( EV-SCC-18、AMC-HN-8和 HCC345)中 KCNQ1OT1和 miR-506-3p表达。以 HCC345细胞为研究对象，转染 KCNQ1OT1小干扰 RNA或共转染 KCNQ1OT1小干扰 RNA与 miR-506-3p抑制剂至 HCC345细胞后， MTT法、流式细胞术、 Transwell分别检测细胞增殖、凋亡、迁移和侵袭。双荧光素酶报告基因实验验证 KCNQ1OT1与 miR-506-3p的调控关系。结果喉鳞状细胞癌组织中 KCNQ1OT1表达高于癌旁组织［( 0.81±0.09)比( 0.27±0.08)］， miR-506-3p表达低于癌旁组织［( 0.24±0.08)比( 0.83±0.09)］。喉鳞状细胞癌细胞系( EV-SCC-18、AMC-HN-8和 HCC345)中 KCNQ1OT1表达均高于 WI-38细胞［( 2.44±0.22)、(2.69±0.21)、(3.61±0.24)比( 1.00±0.13)］， miR-506-3p表达均低于 WI-38细胞［( 0.41±0.13)、(0.30±0.12)、 |
| 英文摘要: |
| Objective To investigate the effect and mechanism of LncRNA KCNQ1OT1 on the biological behavior of laryngeal squa-mous cell carcinoma cells.Methods From May 2019 to February 2020, the expression levels of KCNQ1OT1 and miR-506-3p in 45 cas-es of cancer tissues and para-cancerous tissues from patients with laryngeal squamous cell carcinoma from the Huantai Branch of Shan-dong University Qilu Hospital, and human embryonic lung fibroblasts WI-38 purchased from Shanghai Yansheng Industrial Co., Ltd. and laryngeal squamous cell carcinoma cell lines (EV-SCC-18, AMC-HN-8 and HCC345) purchased from Shanghai Cell Bank of the Chinese Academy of Sciences were detected by RT-qPCR. HCC345 cells were used as the research object and transfected with KCNQ1OT1 small interfering RNA or co-transfected with KCNQ1OT1 small interfering RNA and miR-506-3p inhibitor. MTT, flow cytometry and Transwellwere used to detect the proliferation, apoptosis, migration and invasion. The double luciferase reporter gene experiment verified the regu-latory relationship between KCNQ1OT1 and miR-506-3p.Results The expression of KCNQ1OT1 in laryngeal squamous cell carcinoma was higher than that in adjacent tissues [(0.81±0.09) vs. (0.27±0.08)], but the expression of miR-506-3p was decreased lower than that in adjacent tissues [(0.24±0.08) vs. (0.83±0.09)]. The expression of KCNQ1OT1 in laryngeal squamous cell carcinoma cell lines (EV-SCC-18, AMC-HN-8 and HCC345) were higher than that in WI-38 cells [(2.44±0.22), (2.69±0.21), (3.61±0.24) vs. (1.00±0.13)], but the expres-sion of miR-506-3p were lower than that of WI-38 cells [(0.41±0.13), (0.30±0.12), (0.22±0.11) vs. (1.00±0.12)]. Compared with HCC345 cells without knocking down KCNQ1OT1, the viability [(0.41±0.06) vs. (0.81±0.12)], the number of migration [(86.32±15.21) vs. (162.31± 20.23)], and the number of invasion [(65.23±12.05) vs. (140.26±18.27)] of HCC345 cells that knocked down KCNQ1OT1 were reduced, but cell apoptosis was increased [(34.13±3.60)% vs. (3.79±2.37)%]. KCNQ1OT1 negatively regulated miR-506-3p in HCC345 cells. Knockdown of miR-506-3p reversed the effect of knocking down KCNQ1OT1 on the proliferation, apoptosis, migration and invasion of HCC345 cells.Conclusion KCNQ1OT1 is highly expressed in laryngeal squamous cell carcinoma tissues and cell lines. It promotes themalignant biological behavior of laryngeal squamous cell carcinoma cells by targeting the expression of miR-506-3p. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
