| 谭莉霞,杨文义,闫春晓,等.长链非编码 RNA小核仁 RNA宿主基因 4在胃癌中的表达及与预后的关系[J].安徽医药,2022,26(3):470-474. |
| 长链非编码 RNA小核仁 RNA宿主基因 4在胃癌中的表达及与预后的关系 |
| Expression of LncRNA small nucleolar RNA host gene 4 in gastric cancer and its relationship with prognosis |
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| DOI：10.3969/j.issn.1009-6469.2022.03.011 |
| 中文关键词: 胃肿瘤 长链非编码 RNA 小核仁 RNA宿主基因 4 肿瘤侵润 肿瘤分期 预后 |
| 英文关键词: Stomach neoplasms Long non-coding RNA Small nucleolar RNA host gene 4 Neoplasm invasiveness Neoplasm staging Prognosis |
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| 中文摘要: |
| 目的探究长链非编码 RNA(LncRNA)小核仁 RNA宿主基因 4(SNHG4)在胃癌中的表达以及与预后的关系。方法选取 2015年 1月至 2016年 10月于河南大学第一附属医院肿瘤科行手术根治术切除的 113例胃癌组织及 82例癌旁正常胃黏膜组织为研究对象，利用实时荧光定量逆转录聚合酶链反应( qRT-PCR)检测 LncRNA SNHG4表达水平。对所有病人术后进行电话或门诊随访，统计胃癌病人生存时间，绘制 Kaplan-Meier生存曲线，组间生存曲线差异用 log-rank检验；多因素 Cox回归分析影响胃癌病人预后不良的危险因素分析。结果癌症基因组图谱( TCGA)数据库显示， SNHG4基因在 415例胃癌组织中的表达水平为( 1.95±0.62)明显高于其在 34例正常胃黏膜组织中的表达水平( 0.43±0.12)(t=14.26，P<0.001)； LncRNA SNHG4在 113例胃癌组织中的表，达水平( 1.67±0.64)明显高于其在 82例癌旁正常胃黏膜组织中的表达水平( 0.98±0.12)(t=9.63，P<0.001)； LncRNA SNHG4水平高低与胃癌病人临床分期、肿瘤分化程度、浸润深度、淋巴结是否转移有关( P<0.05)；单因素分析显示，临床分期、肿瘤分化程度、浸润深度、淋巴结转移与终点事件发生有关( P<0.05)；生存分析结果显示， LncRNA SNHG4高表达组胃癌病人 5年累积生存率为 28.85%，中位生存时间 16.0个月； LncRNA SNHG4低表达组胃癌病人 5年累积生存率为 |
| 英文摘要: |
| Objective To investigate the expression of long non-coding RNA (LncRNA) small nucleolar RNA host gene 4 (SNHG4) in gastric cancer and its relationship with prognosis.Methods Totally 113 samples of gastric cancer tissues and 82 samples of normalgastric mucosa tissues, obtained from radical operations in Oncology Department of The First Affiliated Hospital of Henan Universityfrom January 2015 to October 2016, were selected as the research objects, and the expression level of LncRNA SNHG4 was detected byreal-time fluorescent quantitative reverse transcription polymerase chain reaction (qRT-PCR). All patients were followed up by telephone or clinic. The survival time of gastric cancer patients was counted, Kaplan-Meier survival curve was drawn, and the difference in survival curve between groups was analyzed by log-rank test; multivariate Cox regression analysis was performed to analyze the risk factors of poor prognosis in patients with gastric cancer.Results The cancer genome atlas (TCGA) database showed that the expressionlevel of SNHG4 in 415 samples of gastric cancer tissues was (1.95±0.62), which was significantly higher than that in 34 samples of normal gastric mucosa tissues [(0.43±0.12), t = 14.26, P < 0.001]; the expression level of LncRNA SNHG4 in 113 gastric cancer tissueswas significantly higher than that in 82 normal gastric mucosa tissues [(1.67±0.64) vs. (0.98±0.12), t = 9.63, P < 0.001]. The level of LncRNA SNHG4 was correlated with clinical stage, tumor differentiation, depth of invasion and lymph node metastasis (P < 0.05); univariate analysis results showed that clinical stage, tumor differentiation, depth of invasion and lymph node metastasis were related to the occurrence of end-point events (P < 0.05). Survival analysis results showed that, the 5-year cumulative survival rate of gastric cancer patients in the LncRNA SNHG4 high expression group was 28.85%, and the median survival time was 16.0 months; the 5-year cumulativesurvival rate of gastric cancer patients in the LncRNA SNHG4 low expression group was 42.62%, and the median survival time was 27.0 months; the difference in log-rank test was statistically significant (χ2= 11.05, P < 0.001). Multivariate Cox regression analysis results showed that clinical stage Ⅲ-Ⅳ, low degree of differentiation, depth of invasion T3-T4, lymph node metastasis and high expression of LncRNA SNHG4 were independent predictors of poor prognosis in patients with gastric cancer (P < 0.05).Conclusion The expression of LncRNA SNHG4 is high in gastric cancer tissues, and it is an independent predictor of poor prognosis in gastric cancer patients. |
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