| 李海霞,李慧朋,王文文.卵巢早衰病人血清微小 RNA-125b表达及与中医证型的相关性分析[J].安徽医药,2022,26(3):509-513. |
| 卵巢早衰病人血清微小 RNA-125b表达及与中医证型的相关性分析 |
| Analysis of correlations between serum microRNA-125b and TCM syndromes in patients with premature ovarian failure |
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| DOI：10.3969/j.issn.1009-6469.2022.03.020 |
| 中文关键词: 原发性卵巢功能不全 微小 RNA-125b 中医证型 相关性 |
| 英文关键词: Primary ovarian insufficiency MiR-125b TCM syndrome Correlation |
| 基金项目:2018年度河南省科技攻关项目( 182102310237) |
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| 中文摘要: |
| 目的探讨卵巢早衰病人血清微小 RNA(miR)-125b表达水平及与中医证型的相关性分析。方法选取 2017年 3月至 2019年 6月郑州市第七人民医院妇科收治的 136例卵巢早衰病人作为研究组，选取同期 120例该院体检健康者作为对照组，观察两组研究对象及不同中医证型血清 miR-125b、抗米勒管激素( AMH)、卵泡刺激素( FSH)/黄体生成素( LH)水平变化，分析 miR-125b表达水平与 AMH、FSH/LH、中医证型的关系。结果卵巢早衰病人以肾虚肝郁证( 41.18%)、肝肾阴虚证( 23.53%)、肾虚血瘀证( 16.91%)、脾肾阳虚证( 14.70%)较为常见；研究组病人血清 AMH水平［(1.98±0.64)pmol/L比( 10.95±2.62)pmol/L］明显低于对照组( P<0.05)，miR-125b［(2.67±0.91)比( 1.01±0.29)］、 FSH［(47.28±9.15)U/L比( 6.05±1.02)U/L］、 LH［(30.46±8.24) U/L比( 5.32±1.37)U/L］、 FSH/LH比值［( 1.62±0.52)比( 1.05±0.31)］明显高于对照组( P<0.05)；肝肾阴虚证组病人血清 AMH水平低于肾虚肝郁证、肾虚血瘀证、脾肾阳虚证病人( P<0.05)脾肾阳虚证组低于肾虚血瘀证组( P<0.05)；肝肾阴虚证组病人 miR-125b、FSH、LH水平高于肾虚肝郁证、肾虚血瘀证、脾肾阳虚，证病人( P<0.05)脾肾阳虚证组 miR-125b、FSH、LH水平高于肾虚血瘀证、肾虚肝郁证组( P<0.05)；肝肾阴虚证组病人 FSH/LH比值低于肾虚肝郁，证、肾虚血瘀证病人( P<0.05)，脾肾阳虚证组低于肾虚肝郁证病人( P<0.05)；卵巢早衰病人血清 miR-125b表达水平与 AMH水平呈负相关( P<0.05)与 FSH/LH比值呈正相关( P<0.05)；血清 AMH与肾虚肝郁证、肝肾阴虚证、脾肾阳虚证呈负相关( P<0.05)，miR-125b、FSH/LH比，值与肾虚肝郁证、肝肾阴虚证、肾虚血瘀证、脾肾阳虚证呈正相关( P<0.05)。结论卵巢早衰病人血清 miR-125b高表达，与 AMH水平呈负相关，与 FSH/LH比值呈正相关，且肝肾阴虚证、脾肾阳虚证病人血清 miR-125b水平变化较为明显，血清 AMH与肝肾阴虚证、脾肾阳虚证呈负相关。 |
| 英文摘要: |
| Objective To investigate the expression level of serum microRNA (miR)-125b in patients with premature ovarian failure (POF) and its correlation with traditional Chinese medicine syndromes.Methods A total of 136 patients with POF who were admittedto the Department of Gynecology of the 7th People′s Hospital of Zhengzhou from March 2017 to June 2019 were selected for the study,and 120 healthy people with physical examination in the hospital during the same period were selected as the control group. Changes inserum miR-125b, anti-Mullerian hormone (AMH), follicle-stimulating hormone (FSH)/luteinizing hormone (LH) in the two groups anddifferent TCM syndromes were observed, and the correlations between miR-125b and AMH, FSH/LH, and TCM syndromes were analyzed.Results In patients with POF patients, kidney deficiency and liver stasis syndrome (41.18%), Yang deficiency of liver and kidney syndrome (23.53%), kidney deficiency and blood stasis syndrome (16.91%) and Yang deficiency of spleen and kidney syndrome(14.70%) were more common; the serum AMH level in patients in the study group was significantly lower than that in the control group[(1.98±0.64) pmol/L vs. (10.95±2.62) pmol/L] (P < 0.05), while miR-125b [(2.67±0.91) pmol/L vs. (1.01±0.29) pmol/L], FSH [(47.28± 9.15) pmol/L vs. (6.05±1.02) pmol/L], LH [(30.46±8.24) pmol/L vs. (5.32±1.37) pmol/L] levels and the FSH/LH ratio [(1.62±0.52) pmol/ L vs. (1.05±0.31) pmol/L] was significantly higher than that of the control group (P < 0.05); the serum AMH level of the patients withYang deficiency of liver and kidney syndrome was lower than that of patients with kidney deficiency and liver stasis syndrome, kidneydeficiency and blood stasis syndrome, and Yang deficiency of spleen and kidney syndrome (P < 0.05), and the serum AMH level of thepatients with kidney deficiency and blood stasis syndrome was lower than that of the patients with Yang deficiency of spleen and kidneysyndrome (P < 0.05); the levels of miR-125b, FSH, and LH in patients with Yang deficiency of liver and kidney syndrome were higherthan those in patients with kidney deficiency and liver stasis syndrome, kidney deficiency and blood stasis syndrome, and Yang deficiency of spleen and kidney syndrome(P<0.05), and the levels of miR-125b, FSH and LH in the Yang deficiency of spleen and kidneysyndrome group was higher than those in the kidney deficiency and blood stasis syndrome group and the kidney deficiency and liver stasis syndrome group (P<0.05); the ratio of FSH/LH in patients with Yang deficiency of liver and kidney syndrome was lower than that inthe kidney deficiency and liver stasis syndrome and kidney deficiency and blood stasis syndrome group(P < 0.05), while that in Yangdeficiency of spleen and kidney syndrome group was lower than that in patients with of kidney deficiency and liver stasis syndrome (P < 0.05); the serum miR-125b level was negatively correlated with AMH (P < 0.05), and positively correlated with the ratio of FSH/LH in POF patients (P < 0.05); the serum AMH in POF patients was negatively correlated with kidney deficiency and liver stasis syndrome,Yang deficiency of liver and kidney syndrome and Yang deficiency of spleen and kidney syndrome (P < 0.05), and the serum miR-125b level and FSH/LH ratio was positively correlated with kidney deficiency and liver stasis syndrome, Yang deficiency of liver and kidneysyndrome, kidney deficiency and blood stasis syndrome and Yang deficiency of spleen and kidney syndrome (P < 0.05).Conclusions Serum miR-125b is highly expressed in POF patients and is negatively correlated with AMH levels and positively correlated with theFSH/LH ratio. The changes in serum miR-125b levels in patients with Yang deficiency of liver and kidney syndrome and Yang deficiency of spleen and kidney syndrome are more significant. Serum AMH is negatively correlated with Yang deficiency of liver and kidneysyndrome and Yang deficiency of spleen and kidney syndrome. |
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