| 席羽,周冬梅,赵锛活,等.二磷酸腺苷核糖基化因子6 对甲状腺乳头状癌细胞增殖和转移的影响[J].安徽医药,2022,26(5):959-964. |
| 二磷酸腺苷核糖基化因子6 对甲状腺乳头状癌细胞增殖和转移的影响 |
| Effects and mechanisms of ARF6 on proliferation and metastasis of papillary thyroid carcinoma cells |
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| DOI：10.3969/j.issn.1009-6469.2022.05.026 |
| 中文关键词: 甲状腺肿瘤 癌，乳头状 二磷酸腺苷核糖基化因子6 细胞外调节激酶1/2 上皮型钙黏蛋白 基质金属蛋白酶-9 |
| 英文关键词: Thyroid neoplasms Carcinoma,papillary ARF6 Extracellular signal-regulated kinase 1/2 E-cadherin Matrix metalloproteinase-9 |
| 基金项目: |
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| 中文摘要: |
| 目的探讨二磷酸腺苷核糖基化因子6(ARF6)对甲状腺乳头状癌细胞B-CPAP增殖和转移的影响及相关机制。方法在B-CPAP细胞中构建ARF6沉默细胞系及瞬时转染外源3×Flag-ARF6；利用EdU、CCK-8实验检测ARF6对B-CPAP细胞增殖的影响；利用Transwell实验检测ARF6对B-CPAP细胞转移的影响；利用蛋白质印迹法(Western blotting)检测沉默及过表达ARF6对细胞外调节激酶1/2(ERK1/2)、上皮型钙黏蛋白(E-cadherin)及基质金属蛋白酶-9(MMP-9)蛋白表达的影响。结果与对照组相比，沉默ARF6后B-CPAP细胞的增殖、侵袭和迁移的能力明显下降［ARF6沉默组及其对照组EdU阳性细胞的比例分别为(33.75±1.50)％和(39.65±2.21)％，第5天OD值分别为(0.66±0.03)和(0.83±0.03)，细胞侵袭数量分别为(614.66±18.52)和(898.34±38.94)个，细胞迁移数量分别为(588.67±18.02)和(922.32±34.91)个，均P<0.05］。与对照组相比，过表达ARF6后B-CPAP细胞的增殖、侵袭和迁移的能力明显提升［ARF6过表达组及其对照组EdU阳性细胞的比例分别为(46. 53±4.09)％和(36.89±2.27)％，第5 天OD 值分别为(1.06±0.03)和(0.81±0.02)，细胞侵袭数量分别为(943.68±24.69)和(758. 42±10.78)个，细胞迁移数量分别为(974.68±23.68)和(726.32±27.04)个，均P <0.05］。沉默ARF6可上调B-CPAP细胞中p-ERK1/2和MMP-9的表达、下调E-cadherin的表达，过表达ARF6时则相反，均P<0.05。结论ARF6可能通过调控p-ERK1/2、MMP-9及E-cadherin的蛋白表达，从而促进甲状腺乳头状癌细胞B-CPAP的增殖和转移。 |
| 英文摘要: |
| Objective To investigate the effects of ADP-ribosylation factor 6 (ARF6) on proliferation and metastasis of papillary thy?roid carcinoma B-CPAP cells and its related mechanism.Methods ARF6 silencing cell line was constructed in B-CPAP cells and the exogenous 3×Flag-ARF6 was transiently transfected. The effects of ARF6 on proliferation of B-CPAP cells were measured by EdU and CCK-8 assays. The effects of ARF6 on metastasis of B-CPAP cells were measured by Transwell assays. Western blotting was used to de?tect the expression of ERK1/2, E-cadherin and MMP-9 while silencing and overexpressing ARF6.Results Compared with control group, silencing ARF6 expression significantly inhibited the proliferation, invasion and migration of papillary thyroid carcinoma BCPAP cells [the proportions of EdU positive cells of ARF6 silencing group and its control group were respectively (33.75±1.50) % and(39.65±2.21)%, the OD values of 5th day were respectively (0.66±0.03) and (0.83±0.03), the numbers of cell invasion were respectively(614.66±18.52) and (898.34±38.94), the numbers of cell migration were respectively (588.67±18.02) and (922.32±34.91), all P<0.05].Compared with control group, over-expression of ARF6 significantly promoted the proliferation, invasion and migration of papillary thy?roid carcinoma B-CPAP cells [the proportions of EdU positive cells of ARF6 over-expressing group and its control group were respec?tively (46. 53±4.09)% and (36.89±2.27)%, the OD values of 5th day were respectively (1.06±0.03) and (0.81±0.02), the numbers of cell invasion were respectively (943.68±24.69) and (758. 42±10.78), the numbers of cell migration were respectively (974.68±23.68) and (726.32±27.04), all P<0.05]. ARF6 positively regulated the expression of p-ERK1/2 and MMP-9 and negatively regulated the expres?sion of E-cadherin, but the opposite was true when ARF6 was overexpressed, all P<0.05.Conclusion ARF6 may promote the prolifer?ation and metastasis of papillary thyroid carcinoma B-CPAP cells by regulating the expression of p-ERK1/2, MMP-9 and E-cadherin. |
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