| 刘亚,贾超,范秋玉,等.系统性红斑狼疮病人外周血B 细胞中CD72 的表达及临床意义[J].安徽医药,2022,26(5):1008-1011. |
| 系统性红斑狼疮病人外周血B 细胞中CD72 的表达及临床意义 |
| CD72 expression in peripheral blood B cells of patients with systemic lupus erythematosus and its clinical significance |
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| DOI：10.3969/j.issn.1009-6469.2022.05.037 |
| 中文关键词: 系统性红斑狼疮 B淋巴细胞 CD72 SLE疾病活动指数评分 P-JNK |
| 英文关键词: Systemic lupus erythematosus B lymphocytes CD72 SLE disease activity index score P-JNK |
| 基金项目:武汉市卫生健康委员会医学科研项目(WX19Y11) |
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| 中文摘要: |
| 目的检测系统性红斑狼疮(SLE)病人外周血免疫B淋巴细胞中CD72蛋白的表达及临床意义并探究其下游P-JNK的表达，讨论其在SLE发病中的意义。方法选取2016年1月至2017年12月武汉市第一医院收治的SLE病人48例为观察组，根据选取年龄、性别相匹配于武汉市第一医院健康体检的正常人48例为健康对照组，使用流式细胞术检测和免疫酶联法比较两组研究对象采集外周血B淋巴细胞CD72及P-JNK含量表达，同时分析B淋巴细胞CD72表达与SLE疾病活动指数评分之间的关系。结果观察组SLE外周血B淋巴细胞数量［(781±134)个比(277±65)个］、CD72下游的P-JNK含量明显高于健康对照组［(22.67±3.01)ng/L比(18.25±2.67)ng/L］、CD72阳性率［(58.7±9.9)%比(69.5±10.7)%］低于健康对照组，同时CD72阳性率与SLE疾病活动指数评分之间具有负性相关关系(P<0.05)。结论SLE病人外周的B淋巴细胞CD72阳性率较低，同时其与病人的SLE疾病活动指数评分具有负性相关性，可能与CD72通过其P-JNK介导的病理过程相关，可辅助临床SLE的病情的诊断及评估。 |
| 英文摘要: |
| Objective To detect the expression of CD72 protein in peripheral blood immune B lymphocytes of patients with system?ic lupus erythematosus (SLE) explore the expression of P-JNK downstreamtheir significance in the pathogenesis of SLE.Methods For?ty-eight SLE patients treated in the First Hospital of Wuhan from January 2016 to December 2017 were selected as the observation group. Forty-eight normal people were elected whose age and gender matched the health examination in the First Hospital of Wuhan as the control group. Flow cytometrydetection and enzyme-linked immunosorbent assay were used to compare the expression of CD72 and P-JNK in B lymphocytes from peripheral blood collected in all subjects, and the relationship between CD72 content of B lymphocytes and SLE disease activity index score was analyzed.Results Content of B lymphocytes[(781±134)vs. (277±65)] and expression of p-JNK was significantly higher than in healthy control group[(22.67±3.01)ng/L vs. (18.25±2.67)ng/L].CD72 positive rate[(58.7±9.9)% vs.(69.5±10.7)%] of peripheral blood B lymphocytes in SLE was lower than in the control group.Meanwhile, there was a negative correla?tion between the CD72 positive rate and the SLE disease activity index score (P<0.05).Conclusions Peripheral B lymphocytes have alow CD72 positive rate in SLE patientsand it has a negative correlation with the patient's SLE disease activity index score, which may be related to the pathological process mediated by CD72 through its P-JNK, which can assist clinical diagnosis and evaluation of the condition of SLE. |
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