| 宋应周,孟松峰.肝细胞癌的细胞分裂周期基因 20表达与临床病理特征关系及对预后的影响[J].安徽医药,2022,26(6):1222-1225. |
| 肝细胞癌的细胞分裂周期基因 20表达与临床病理特征关系及对预后的影响 |
| The relationship between the expression of cell division cycle gene 20 in hepatocellular carcinoma and the clinicopathological characteristics of patients and its influence on prognosis |
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| DOI：10.3969/j.issn.1009-6469.2022.06.039 |
| 中文关键词: 癌，肝细胞 细胞分裂周期基因 20 病理学，临床 预后 |
| 英文关键词: Carcinoma, hepatocellular Cell division cycle gene 20 Pathology, clinical Prognosis |
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| 中文摘要: |
| 目的分析肝细胞癌病人细胞分裂周期基因 20(CDC20)表达与临床病理特征的关系及对预后的影响。方法收集 2016年 5月至 2017年 12月在商丘市第三人民医院行手术治疗的肝细胞癌病人 146例，实时荧光定量逆转录聚合酶链反应(qRT-PCR)、蛋白质印迹法和免疫组织化学检测肝细胞癌及癌旁组织中 CDC20的表达情况。将免疫免疫组织化学评分 0~7分和 8~12分的病人分别设为 CDC20低水平组( n=86)和 CDC20高水平组( n=60)分析两组间的临床病理特征和生存率。结果 CDC20 mRNA在肝细胞癌组织中相对表达水平为( 2.33±0.67)，高于癌旁组织(0.9，7±0.29)(P<0.001)。 CDC20在肝细胞癌组织中蛋白表达水平为( 0.91±0.05)高于癌旁组织( 0.14±0.05)(P<0.001)。 CDC20在肝细胞癌组织中阳性表达率为 77.4%(113/ 146)高于癌旁组织 8.2%(12/146)(，P<0.001)。 CDC20的表达与病人的肿瘤长径、被膜侵犯、脉管侵犯、肿瘤多发、分化程度和 TNM分，期有关(均 P<0.05)。 CDC20高水平组术后 3年总生存率( 61.7%)和疾病无进展生存率( 50.0%)，均低于 CDC20低水平组(87.2%、69.8%)(均 P<0.05)。结论肝细胞癌中 CDC20的水平增加，可能参与肿瘤的增殖和侵袭。 CDC20高水平提示病人 |
| 英文摘要: |
| Objective To analyze the relationship between the expression of hepatocellular carcinoma cell division cycle gene 20(CDC20) and clinicopathological characteristics of patients and its impact on prognosis.Methods A total of 146 hepatocellular carcinoma patients who underwent surgical treatment in Shangqiu Third People's Hospital from May 2016 to December 2017 were collected.Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), Western blotting and immunohistochemistry were used todetect the expression of CDC20 in hepatocellular carcinoma and adjacent tissues. The patients with immunohistochemical score of 8-12 points were enrolled in CDC20 high leave group (n=60), and the patients with immunohistochemical score of 0-7 points were enrolled in CDC20 low leave group (n=86). The clinicopathological features and survival rates of the two groups were analyzed.Results The relative expression level of CDC20 mRNA in hepatocellular carcinoma tissues was (2.33±0.67), which was higher than that of adjacent tissues(0.97±0.29), (P<0.001). The protein expression level of CDC20 in hepatocellular carcinoma tissue was (0.91±0.05), which washigher than that of adjacent tissues (0.14±0.05) (P<0.001). The positive expression rate of CDC20 in hepatocellular carcinoma tissueswas 77.4% (113/146), which was higher than that in adjacent tissues 8.2% (12/146) (P<0.001). The high expression of CDC20 was related to the patient's maximum tumor diameter, capsule invasion, vascular invasion, tumor frequency, degree of differentiation andTNM stage (all P<0.05). The 3-year overall survival rate (61.7%) and disease progression-free survival rate (50.0%) in the CDC20 high leave group were lower than those in the CDC20 low leave group (87.2%, 69.8%) (All P<0.05).Conclusions The expression level ofCDC20 in hepatocellular carcinoma is increased, which may be involved in tumor proliferation and invasion. The high expression ofCDC20 indicates poor prognosis of patients, and it is expected to become a tumor marker for judging the prognosis of hepatocellular carcinoma. |
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