| Objective To explore the mechanism of salvianolic acid A on lipopolysaccharide (LPS)-induced apoptosis of human glo-merular mesangial cells (HGMCs).Methods The study period was from May 2018 to May 2019. The HGMCs were purchased fromHuatuo Biotechnology and divided into five groups: A group, B group, C group, D group and E group. The cells in group A did not addany drugs, and 10 mg/L LPS was added to the B, C, D and E groups. One hour later, groups C, D, and E were treated with low-dose sal-vianolic acid A (10 mg/L), medium-dose salvianolic acid A (20 mg/L) and high-dose salvianolic acid A (40 mg/L) for 24 h, respectively.Cell proliferation, apoptosis, cell cycle distribution and the expression of phosphatidylinositol-3 (PI3K)/protein kinase (AKT)/mammali-an target of rapamycin (mTOR) signaling pathway indicators were detected.Results Compared with group A, the apoptosis rate and the distribution rate of cells in G0/G1 phase in group B decreased, while the distribution rate of cells in S phase and G2/M phase in-creased.Compared with group B, the distribution rate of cells in G0/G1 phase and apoptosis rate in groups C, D, and E increased andthe distribution rate of cells in S phase and G2/M phase decreased. Compared with group C, the apoptosis rate and the distribution rateof cells in G0/G1 phase in groups D and E increased, and the distribution rate of cells in S phase and G2/M phase decreased (All P< 0.05). Compared with group D, the apoptotic rate of cells in group E was (37.59±4.62)%, and the distribution rate of cells in G0/G1phase was (86.95±11.62)%, and the distribution rates of cells in S phase and G2/M phase were (10.23±1.03)% and (4.26±1.03)%, re-spectively (P < 0.05). Compared with group A, the cell proliferation rate, p-PI3K, p-AKT, p-mTOR, caspase-3, and B-cell lymphoma/ leukemia-2 (Bcl-2) were increased, and the expression of B-cell lymphoma/leukemia-associated protein X (Bax) was decreased (P < 0.05). Compared with group B, the cell proliferation rate and p-PI3K, p-AKT, p-mTOR, caspase-3 and Bcl-2 expression in groups C, D, and E decreased, while the expression of Bax increased (P < 0.05). Compared with group C, the cell proliferation rate and p-PI3K, p-AKT, p-mTOR, caspase-3 and Bcl-2 expression in groups D and E decreased, while the expression of Bax increased (P < 0.05). Com-pared with group D of the 12 h proliferation rate, the expression of p-PI3K, p-Akt, p-mTOR, caspase-3, Bcl-2 and Bax [(26.12±2.25)%,(0.49±0.28), (0.87±0.45), (0.59±0.25), (0.88±0.12), (1.25±0.20) and (0.87±0.12)], Group E were (19.02±1.06)%, (0.25±0.11), (0.32±0.15), (0.25±0.12), (0.54±0.12), (0.42±0.22), and (1.59±0.22), respectively (P < 0.05).Conclusions Salvianolic acid A may regulate the apoptosis-related proteins caspase-3, Bcl-2 and Bax by promoting the inactivation of the PI3K/AKT/mTOR signaling pathway and play a role in promoting LPS-induced apoptosis of human glomerular mesangial cells, inhibiting proliferation, and inhibiting apoptosis.The role of arresting cell cycle progression. |
