| 万全会,徐晓宁,王刘玉,等.长链非编码 RNA-ROR在骨肉瘤组织中表达及对预后的影响[J].安徽医药,2022,26(7):1332-1335. |
| 长链非编码 RNA-ROR在骨肉瘤组织中表达及对预后的影响 |
| Expression of long non-coding RNA-ROR in osteosarcoma tissues and its influence on prognosis |
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| DOI：10.3969/j.issn.1009-6469.2022.07.014 |
| 中文关键词: 骨肉瘤 长链非编码 RNA-重编程调节器 生存分析 Cox回归分析 |
| 英文关键词: Osteosarcoma Long non-coding RNA-reprogramming Survival analysis Cox regression analysis |
| 基金项目:2019年河南省科技攻关计划( LHGJ20190168) |
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| 中文摘要: |
| 目的探讨长链非编码 -重编程调节器(LncRNA-ROR)在骨肉瘤组织中表达及对病人预后的影响。方法选取南阳市第二人民医院 2008年 2月至 2014年 5月行手术切除的原发性骨肉瘤病人 96例，采用实时荧光定量 PCR术分析骨肉瘤和正常骨组织中 LncRNA-ROR表达水平，所有病人术后随访，随访时间范围 7～60个月，记录病人生存时间，获得病人 5年生存率。使用 Kaplan-Meier法开展生存分析，使用 Cox回归模型分析生存时间的相关影响因素。结果 LncRNA-ROR在骨肉瘤组织中表达量为(2.31±0.20)，高于正常骨组织的(1.03±0.11)(t=39.94，P<0.001)； LncRNA-ROR在不同 Enneking分期、 KPS评分及是否发生远处转移间的表达量差异有统计学意义(P<0.05)；生存分析显示，低表达组病人中位生存时间 51.00个月，生存时间( 45.42±3.26)个月， 5年生存率 50.00%，均高于高表达组病人，分别为 21.00个月、(27.31±2.04)个月和 18.57%，差异有统计学意义( χ2=13.20，P<0.001)； Enneking分期、远处转移和 LncRNA-ROR表达是影响骨肉瘤病人 5年生存率的独立风险因素( HR=2.15、2.83和 2.09，P<0.05)。结论 LncRNA-ROR在骨肉瘤组织中表达量升高，且与不良预后有关，是病人生存时间的独立影响因素。 |
| 英文摘要: |
| Objective To investigate the expression of long non-coding RNA-regulator of reprogramming (LncRNA-ROR) in osteo-sarcoma tissues and its influence on prognosis of patients.Methods A total of 96 cases of patients with primary osteosarcoma who un-derwent surgical resection in Nanyang Second People's Hospital from February 2008 to May 2014 were selected. The expression levelsof LncRNA-ROR in osteosarcoma and normal bone tissues were detected by real-time fluorescent quantitative PCR. All patients were followed up after surgery. And the follow-up time ranged from 7 to 60 months. The survival times of the patients were recorded, and the 5-year survival rate of the patients was obtained. Kaplan-Meier method was used to analyze the survival analysis. The Cox regressionmodel was used to analyze the related influencing factors of survival time.Results The relative expression level of LncRNA-ROR in osteosarcoma tissues was (2.31±0.20), which was higher than that of normal bone tissue, which was (1.03±0.11) (t=39.94, P<0.001). The differences of the expression levels of LncRNA-ROR among different Enneking stages, KPS scores and whether distant metastasis occurred were statistically significant (P<0.05). Survival analysis showed that the median survival time, average survival time and the 5-year survival rate in the low expression group were 51.00 months, (45.42±3.26) months and 50.00%, respectively, which were higherthan those in the high expression group, which was 21.00 months, (27.31±2.04) months and 18.57% (χ2=13.20, P<0.001). Cox propor-tional hazard regression model showed that Enneking stage, distant metastasis and LncRNA-ROR expression were independent risk fac-tors affecting 5-year survival rate of osteosarcoma patients (HR=2.15, 2.83 and 2.09, P<0.05).Conclusions The expression level of Ln-cRNA-ROR expressed in osteosarcoma tissues is increased. It is related with poor prognosis, and is an independent factor affecting thesurvival time of patients. |
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