| 王航宇,杨文义,韩大正,等.老年溃疡性结肠炎病人受体蛋白 6、降钙素原和白细胞介素 -18表达水平及意义分析[J].安徽医药,2022,26(7):1381-1385. |
| 老年溃疡性结肠炎病人受体蛋白 6、降钙素原和白细胞介素 -18表达水平及意义分析 |
| Expression levels and significance of receptor protein 6, procalcitonin and interleukin-18 in elderly patients with ulcerative colitis |
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| DOI：10.3969/j.issn.1009-6469.2022.07.025 |
| 中文关键词: 结肠炎，溃疡性 老年人 受体蛋白 6 降钙素原 白细胞介素 -18 |
| 英文关键词: Colitis,ulcerative Elderly Receptor protein 6 Procalcitonin Interleukin-18 |
| 基金项目:开封市科技攻关计划项目( 1903099) |
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| 中文摘要: |
| 目的探究老年溃疡性结肠炎(UC)病人核苷酸结合寡聚化结构域样受体蛋白 6(NLRP-6)、降钙素原(PCT)和白细胞介素-18(IL-18)表达水平及意义。方法选取 2019年 1月至 2020年 12月河南大学第一附属医院收治的 72例老年 UC病人，活动期 52例，缓解期 20例(轻度 18例、中度 20例、重度 14例)免疫组化法检测肠黏膜 NLRP-6蛋白表达，荧光定量 PCR法检测肠黏膜 NLRP-6、IL-18基因表达，酶联免疫吸附法检测血清 IL-18，，双抗夹心免疫化学发光法检测血清 PCT水平，评估临床意义。结果缓解期病人 NLRP-6基因和蛋白表达分别为( 0.88±0.29)和( 0.025±0.006)均高于活动期的( 0.58±0.25)和( 0.012±0.004)，而缓解期病人 IL-18基因表达、血清 IL-18、血清 PCT水平分别为(0.15±0.04)、(101.2，4±17.16)ng/L和［0.13(0.13，0.17)］μg/L，均低于活动期的( 0.40±0.26)、(198.04±110.53)ng/L和( 1.11±0.38)μg/L(均 P<0.05)；活动期重度病人 NLRP-6基因和蛋白表达分别为( 0.31± |
| 英文摘要: |
| Objective To investigate the expression levels and significance of nucleotide-binding oligomerization domain-like recep-tor protein 6 (NLRP-6), procalcitonin (PCT) and interleukin-18 (IL-18) in elderly patients with ulcerative colitis (UC).Methods A to-tal of 72 elderly UC patients admitted to the First Affiliated Hospital of Henan University from January 2019 to December 2020 were se-lected, including 52 cases (18 mild, 20 moderate, and 14 severe) in the active stage and 20 cases in the remission stage. The expressionof NLRP-6 protein in intestinal mucosa was detected by immunohistochemistry, and the expression of NLRP-6 and IL-18 genes in intes-tinal mucosa was detected by fluorescence quantitative PCR. Serum IL-18 was detected by enzyme-linked immunosorbent assay, and serum PCT levels were detected by the double-antibody sandwich immunochemiluminescence method to evaluate the clinical signifi-cance.Results The gene and protein expression levels of NLRP-6 in patients in the remission stage were 0.88±0.29 and 0.025±0.006,respectively, which were higher than those in the active stage (0.58 ± 0.25) and (0.012 ± 0.004). The gene levels of IL-18, and serum IL-18 and PCTs in remission stage were 0.15 ± 0.04, 101.24 ± 17.16 ng/L and 0.13 (0.13, 0.17) μg/L, respectively, which were lower thanthose in the active stage (0.40 ± 0.26), (198.04 ± 110.53) ng/L and (1.11 ± 0.38) μg/L, all of which were significantly different (P < 0.05). The expression levels of the NLRP-6 gene and protein of in severe patients at active stage were 0.31±0.15 and 0.007±0.002, re-spectively, and those in moderate patients were 0.56±0.17 and 0.012±0.002, respectively, which were lower than those in mild patients(0.82±0.15) and (0.015±0.013), respectively. The difference was statistically significant (P < 0.05). The expression of IL-18 gene, serum IL-18 and serum PCT levels in severe patients at active stage were 0.56±0.32, 370.55±54.39 ng/L and 1.43±0.23 μg/L, respectively, inmoderate patients (0.42 ± 0.22), 142.36 (133.34, 162.12) ng/L and 0.13 (1.25 ± 0.17) μg/L, which were higher than those of mild pa-tients (0.26 ± 0.14), (124.34 ± 14.29) ng/L and (0.71 ± 0.28) μg/L], the difference was statistically significant (P< 0.05); the expression of NLRP-6 gene and protein in severe patients was lower than that in moderate patients, and the expression of IL-18 gene, serum IL-18 and serum PCT levels were higher than those in moderate patients, and the difference was statistically significant (P < 0.05); NLRP-6 gene and protein expression were negatively correlated with IL-18 gene expression, serum IL-18, PCT and disease activity in active pa-tients; IL-18 gene expression was positively correlated with serum IL-18, PCT and disease activity, and serum IL-18 was positively corre-lated with serum PCT and disease activity, serum PCT was positively correlated with disease activity ; The AUC(95%CI) of NLRP-6 gene and protein expression, IL-18 gene expression, serum IL-18, and serum PCT to evaluate the moderate to severe UC active stage were 0.92 (0.82-0.98), 0.90(0.79-0.97), and 0.74(0.60-0.85), 0.86(0.74-0.94), 0.98(0.90-1.00), respectively. The evaluation value of the combined application of serum IL-18 and PCT was higher than that of each index alone, and the AUC(95%CI) was 0.99(0.92-1.00).Conclusion The expression of NLRP-6 in elderly patients with active UC was lower than that in patients in remission, while the expression levels of PCT and IL-18 were increased. The detection of the three levels can be used to guide the assessment of disease activity. |
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