| 袁君,王晓娟,王春,等.白芍总苷对胶原性关节炎大鼠血糖和葡萄糖转运体的影响[J].安徽医药,2022,26(8):1505-1509. |
| 白芍总苷对胶原性关节炎大鼠血糖和葡萄糖转运体的影响 |
| Effect of TGP on the blood glucose and glucose transporter of collagen arthritis rats |
| |
| DOI：10.3969/j.issn.1009-6469.2022.08.005 |
| 中文关键词: 关节炎，实验性 白芍总苷 血糖 胰岛素 葡萄糖转运体 |
| 英文关键词: Arthritis,experimental Total glucosides of paeony Blood glucose Insulin Glucose transporter |
| 基金项目:淮南市科技计划项目( 2018B54) |
|
| 摘要点击次数: 888 |
| 全文下载次数: 331 |
| 中文摘要: |
| 目的研究白芍总苷( TGP)对胶原性关节炎大鼠血糖和葡萄糖转运体的调控作用。方法将 30只大鼠采用随机数字表法分为正常组、模型组、 TGP组和甲氨蝶呤( MTX)组。采用在大鼠右后足跖和尾根部皮下注射弗氏完全佐剂和鸡 Ⅱ型胶原诱导胶原性关节炎。大鼠致炎后第 14天( D14)TGP组大鼠按照每天 50 mg/kg，MTX组按照每 3天 0.5 mg/kg，正常组和模型组给予等量溶媒，各组连续灌胃给药 16 d。在大鼠致，炎前和致炎后的不同时间点( D14-D30)观察大鼠足爪肿胀；大鼠致炎前和致炎后的 D14和 D30测量大鼠血糖浓度。末次给药( D30)结束后，酶联免疫吸附法检测血液中胰岛素水平和糖化血红蛋白水平，后处死大鼠，观察踝关节病理，蛋白质印迹法检测大鼠肝组织葡萄糖转运体 -2(GLUT-2)和肌肉组织 GLUT-4水平。结果与正常组相比，模型组大鼠足爪明显肿胀( P<0.01)模型组大鼠踝关节病理评分明显增高( P<0.01)；与模型组相比， TGP组关节肿胀水平明显低于模型组，从 D22起差异有统计学，意义( P<0.01)而 MTX组从 D18起差异有统计学意义， TGP组与 MTX组大鼠踝关节病理评分均低于模型组( P<0.01)。与正常组相比较，模型组，大鼠空腹血糖水平明显升高( P<0.01)，经 TGP给药后，关节炎大鼠血糖水平降为( 9.97±1.13)mmol/L，差异有统计学意义( P<0.01)。与正常组相比较，模型组大鼠空腹胰岛素水平与糖化血红蛋白所占总蛋白比例明显升高(P<0.01)经 TGP给药后，关节炎大鼠胰岛素水平降为(16.7±1.87)μg/L，糖化血红蛋白比例降为( 0.63±0.07)%，差异有统计学意义( P<0.0，1)。与正常组相比，模型组大鼠空腹肌糖原与肝糖原水平明显降低( P< |
| 英文摘要: |
| Objective To study the regulatory effect of total glucosides of paeony (TGP) on blood glucose and glucose transporter inrats with collagen arthritis.Methods Thirty rats were randomly assigned into normal group, model group, TGP group and methotrexate(MTX) group. Collagen arthritis was induced by subcutaneous injection of Freund's complete adjuvant and chicken type Ⅱ collagen atthe root of the right hind foot and tail of rats. On the 14th day (D14) after inflammation, rats in TGP group were given 50 mg/kg/day andthose in MTX group were given 0.5 mg/kg/3 days. The normal group and model group were given the same amount of solvent, and eachgroup was given by gavage for 16 days. The paw swelling was observed in the rats before and at different time points after inflammation;the blood glucose was measured in the rats before and at D14 and D30 after the inflammation. After the last medicine administration (atD30), thelevels of insulin and glycosylated hemoglobin in blood were detected by enzyme-linked immunosorbent assay, and then the rats were sacrificed, and ankle pathology was observed and the levels of glucose transporter-2 (GLUT-2) in liver and GLUT-4 in muscle were detected by Western blotting.Results Compared with the normal group, the paws in the model group were significantly swollen (P<0.01), and the pathological score of ankle in the model group was significantly higher (P<0.01). Compared with the model group, thelevel of joint swelling in TGP group was significantly lower than that in the model group; there was significant difference from D22 (P< 0.01), while in MTX group significant difference was seen from D18. The pathological scores of ankle joints in TGP group and MTX group were lower than that in the model group (P<0.01). Compared with the normal group, the fasting blood glucose level of the model group was significantly increased (P<0.01). After administration of TGP, the blood glucose level of arthritis rats decreased to (9.97±1.13) mmol/L; the difference was significant (P<0.01). Compared with the normal group, the fasting insulin level of the model group in- creased significantly (P<0.01). After TGP administration, the level of insulin in arthritis rats decreased to (16.7±1.87) μg/L and the pro-portion of glycosylated hemoglobin decreased to (0.63 ± 0.07) %; the difference was significant (P<0.01). Compared with the normalgroup, the levels of empty muscle glycogen and liver glycogen in the model group decreased significantly (P <0.01). After TGP adminis-tration, the level of muscle glycogen increased to (5.35±0.82) mg/g and the level of liver glycogen increased to (33.83±4.23) mg/g; thedifferences were significant (P<0.01). Compared with the normal group, the levels of liver GLUT-2 and muscle GLUT-4 in the model group decreased significantly (P<0.01). After TGP administration, the levels of GLUT-2 and GLUT-4 were significantly up-regulated compared with the model group; the differences were significant (P<0.01).Conclusion TGP can reduce the hyperglycemia level of col- lagen arthritis rats, which may be related to the upregulation of liver GLUT-2 and muscle GLUT-4 levels. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
