| 黄香春,谭雪萍,廖天志.微小RNA-455-3p 下调TRIM27 对鼻咽癌细胞增殖、凋亡的影响[J].安徽医药,2022,26(8):1632-1636. |
| 微小RNA-455-3p 下调TRIM27 对鼻咽癌细胞增殖、凋亡的影响 |
| MiR-455-3p regulates the proliferation and apoptosis of nasopharyngeal carcinoma cells by down-regulating TRIM27 |
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| DOI：10.3969/j.issn.1009-6469.2022.08.034 |
| 中文关键词: 鼻咽肿瘤 微小RNA-455-3p TRIM27 增殖 凋亡 |
| 英文关键词: Nasopharyngeal neoplasms MiR-455-3p TRIM27 Proliferation Apoptosis |
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| 中文摘要: |
| 目的研究微小RNA-455-3p(miR-455-3p)对鼻咽癌细胞增殖、凋亡的影响及其机制。方法运用实时荧光定量逆转录聚合酶链式反应(qRT-PCR)检测人鼻咽癌细胞5-8F、CNE-1和鼻咽上皮细胞NP69中miR-455-3p的表达；将miR-NC、miR-455-3p mimics、pcDNA3.1、pcDNA3.1- TRIM27、si-NC、si- TRIM27 组、miR-455-3p mimics 和pcDNA3.1、miR-455-3p mimics 和pcDNA3.1-TRIM27通过脂质体试剂转染到5-8F；四甲基偶氮唑盐比色法(MTT)、流式细胞术、蛋白质印迹法(Western blotting)检测细胞的增殖、凋亡和TRIM27蛋白表达；双荧光素酶报告基因检测实验检测细胞中miR-455-3p与TRIM27的靶向关系。结果与鼻咽上皮细胞NP69(1.37±0.06)相比，人鼻咽癌细胞5-8F(0.42±0.02)、CNE-1(0.96±0.05)中miR-455-3p的表达显著降低(F =314.35，P =0.001)。过表达miR-455-3p 可明显抑制5-8F、CNE-1 细胞的增殖，促进其凋亡；miR-455-3p 可抑制野生型TRIM27细胞的荧光活性，且与TRIM27的表达呈负相关；抑制TRIM27可抑制抑制5-8F细胞的增殖，促进凋亡，过表达TRIM27能够逆转miR-455-3p对鼻咽癌细胞5-8F增殖凋亡的作用。结论miR-455-3p能够调控鼻咽癌细胞增殖、凋亡的作用机制与其靶向TRIM27相关，将可为鼻咽癌的治疗提供新靶点。 |
| 英文摘要: |
| Objective To study the effect of microRNA-455-3p (miR-455-3p) on the proliferation and apoptosis of nasopharyngeal carcinoma cells, and to explore its mechanism.Methods Real-time quantitative reverse transcriptase-polymerase chain reaction (qRTPCR)was used to detect the expressions of miR-455-3p in human nasopharyngeal carcinoma cells 5-8F, CNE-1 and nasopharyngeal ep?ithelial cells NP69; miR-NC, miR-455-3p mimics, pcDNA3.1, pcDNA3.1-TRIM27, si-NC, si-TRIM27, miR-455-3p mimics and pcD?NA3.1, miR-455-3p mimics and pcDNA3.1-TRIM27 all were transfected into 5-8F. Methyl thiazolyl tetrazolium (MTT) assay, flow cy?tometry, and Western blotting were used to detect cell proliferation, apoptosis, and TRIM27 protein expression. Dual luciferase reporter assay was used to detect the targeting relationship between miR-455-3p and TRIM27.Results Compared with nasopharyngeal epithe?lial cells NP69, the expressions of miR-455-3p in human nasopharyngeal carcinoma cells 5-8F and CNE-1 were significantly decreased [(0.42±0.02), (0.96±0.05) vs. (1.37±0.06), F =314.35,P =0.001]. Overexpression of miR-455-3p significantly inhibited the proliferation of 5-8F and CNE-1 cells and promoted apoptosis. MiR-455-3p inhibited the fluorescence activity of wild-type TRIM27 cells and was negatively correlated with the expression of TRIM27. Inhibition of TRIM27 inhibited the proliferation of 5-8F cells and promoted apop?tosis. Overexpression of TRIM27 reversed the effect of miR-455-3p on the proliferation and apoptosis of nasopharyngeal carcinoma cells 5-8F.Conclusion MiR-455-3p regulates the proliferation and apoptosis of nasopharyngeal carcinoma cells by targeting TRIM27,which will provide a new target for the treatment of nasopharyngeal carcinoma. |
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