| 王伟,郑水平,罗琴.长链非编码RNA-肺腺癌转移相关转录因子1 联合胃蛋白酶原对早期胃癌的诊断价值[J].安徽医药,2022,26(8):1645-1649. |
| 长链非编码RNA-肺腺癌转移相关转录因子1 联合胃蛋白酶原对早期胃癌的诊断价值 |
| Diagnostic value of serum lncRNA MALAT1 combined with pepsinogen in early gastric cancer |
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| DOI：10.3969/j.issn.1009-6469.2022.08.037 |
| 中文关键词: 胃肿瘤 血清 长链非编码RNA-肺腺癌转移相关转录因子1 胃蛋白酶原 诊断 |
| 英文关键词: Stomach neoplasms Serum Long non-coding RNA metastasis associated lung adenocarcinoma transcript 1 Pep?sinogen Diagnosis |
| 基金项目:荆门市引导性科研计划项目(2018YDKY054) |
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| 中文摘要: |
| 目的检测早期胃癌病人血清中长链非编码RNA-肺腺癌转移相关转录因子1(lncRNA MALAT1)、胃蛋白酶原Ⅰ、Ⅱ(PGⅠ、PGⅡ)水平，并探讨lncRNA MALAT1、PGⅠ、PGⅡ在胃癌早期诊断中的价值。方法选取2017年3月至2019年4月入住荆门市中医医院并经胃镜及病理组织学检查最终确诊为胃癌的病人96例，其中TNM分期Ⅰ~Ⅱ病人67例(早期胃癌组)，Ⅲ~Ⅳ病人29例(进展期胃癌组)；同期选取82例健康体检者作为对照组。采用实时荧光定量PCR(qRT-PCR)法检测血清中ln?cRNA MALAT1水平、胶乳免疫比浊法检测血清PGⅠ和PGⅡ水平；分析不同临床特征胃癌病人血清lncRNA MALAT1、PGⅠ、PGⅡ水平及lncRNA MALAT1与PGⅠ、PGⅡ水平的相关性；分析lncRNA MALAT1、PGⅠ、PGⅡ对早期胃癌的诊断价值。结果不同肿瘤分化、肿瘤大小、阳性淋巴结百分比、神经浸润、血管侵犯的胃癌病人血清lncRNA MALAT1、PGⅠ、PGⅡ水平比较，均差异无统计学意义(P>0.05)；三组性别、年龄、体质量指数、饮酒比例、吸烟比例等比较，均差异无统计学意义(P>0.05)；进展期胃癌组血清中lncRNA MALAT1、PGⅡ水平显著高于早期胃癌组和对照组，PGⅠ水平显著低于早期胃癌组和对照组(P<0.05)；早期胃癌组血清中lncRNA MALAT1、PGⅡ水平显著高于对照组，PGⅠ水平显著低于对照组(P<0.05)；胃癌病人血清中lncRNA MALAT1水平与PGⅡ呈正相关，与PGⅠ呈负相关(P<0.05)；lncRNA MALAT1、PGⅠ、PGⅡ诊断早期胃癌病人的AUC分别为0.770、0.845、0.808，截断值分别为103.026、52.632、20.361 μg/L，此时的特异度分别为89.0%、80.5%、65.9%，灵敏度分别为63.5%、75.0%、83.3%；lncRNA MALAT1、PGⅠ、PGⅡ联合诊断早期胃癌病人的AUC 为0.888，特异度为81.3%，灵敏度为85.4%。结论lncRNA MALAT1、PGⅠ、PGⅡ可能参与胃癌的发病机制，三者联合检测对早期胃癌的诊断有较好的预警价值。 |
| 英文摘要: |
| Objective To detect the levels of serum long non-coding RNA metastasis associated lung adenocarcinoma transcript 1(lncRNA MALAT1), pepsinogen I and Ⅱ (PGI and PGⅡ) in patients with early gastric cancer, and to explore the values of lncRNA MALAT1, PGI and PGⅡ in the early diagnosis of gastric cancer.Methods A total of 96 patients admitted to Jingmen Hospital of Tradi?tional Chinese Medicine from March 2017 to April 2019 and finally diagnosed as gastric cancer by gastroscopy and pathological histolo?gy were selected as the study subjects, among whom 67 patients (early gastric cancer group) were included in TNM stage Ⅰ-Ⅱ and 29 patients (advanced gastric cancer group) were included in TNM stage Ⅲ-Ⅳ; in the same period, 82 healthy people were selected as con?trol group. Real time fluorescence quantitative PCR (QRT PCR) was used to detect the level of lncRNA MALAT1 in serum, and levels of serum PG I and PG Ⅱ were detected by latex immunoturbidimetry; the serum levels of lncRNA MALAT1, PGⅠ, PGⅡ in patients with gastric cancer with different clinical features, and the correlation between lncRNA MALAT1 and PGⅠ, PGⅡ levels were analyzed;the diagnostic values of lncRNA MALAT1, PGI and PGⅡ in early gastric cancer were analyzed.Results There was no significant differ?ence in sex, age, body mass index, proportion of drinking and smoking among the three groups (P>0.05); there was no significant differ?ence in levels of serum lncRNA MALAT1, PGⅠ and PGⅡ among patients with gastric cancer with different tumor differentiation, tu?mor size, percentage of positive lymph nodes, nerve infiltration and vascular invasion (P>0.05); The serum levels of lncRNA MALAT1 and PG Ⅱ in advanced gastric cancer group were significantly higher than those in early gastric cancer group and control group, and PGⅠ level was significantly lower than that in early gastric cancer group and control group (P<0.05); the levels of serum lncRNA MALAT1 and PGⅡ in early gastric cancer group were significantly higher than those in control group, and the level of PGI in early gastric cancer group was significantly lower than that in control group (P<0.05); the serum level of lncRNA MALAT1 was positively correlated with PG Ⅱ and negatively correlated with PGI in patients with early gastric cancer (P<0.05); the AUCs of lncRNA MALAT1, PGI and PGⅡ in the diagnosis of gastric cancer were 0.770, 0.845 and 0.808, respectively, the truncation values were 103.026, 52.632 and 20.361 μg/L,respectively, the specificities were 89.0%, 80.5% and 65.9%, respectively, and the sensitivities were 63.5%, 75.0% and 83.3% respec?tively; the AUC of combination of lncRNA MALAT1, PGⅠ and PGⅡ in the diagnosis of early gastric cancer was 0.888, the specificity was 81.3%, and the sensitivity was 85.4%.Conclusion LncRNA MALAT1, PGI and PGⅡ may be involved in the pathogenesis of ear?ly gastric cancer, and the combined detection of them has a better early warning value in the diagnosis of early gastric cancer. |
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