| 蒋天从,刘志明,谷孝月,等.子痫前期病人长链非编码 RNA H19和长链非编码 RNA HAND2-AS1表达水平与胰岛素抵抗的相关性研究[J].安徽医药,2024,28(1):49-53. |
| 子痫前期病人长链非编码 RNA H19和长链非编码 RNA HAND2-AS1表达水平与胰岛素抵抗的相关性研究 |
| Correlation between the expression levels of LncRNA H19 and LncRNA HAND2-AS1 and insulin resistance in patients with preeclampsia |
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| DOI：10.3969/j.issn.1009-6469.2024.01.011 |
| 中文关键词: 先兆子痫 RNA，长链非编码 LncRNA H19 LncRNA HAND2-AS1 胰岛素抵抗 |
| 英文关键词: Pre-eclampsia RNA, long noncoding LncRNA H19 LncRNA HAND2-AS1 Insulin resistance |
| 基金项目:2022年度河北省医学科学研究项目( 20221770) |
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| 中文摘要: |
| 目的检测子痫前期病人胎盘组织、血清长链非编码 RNA H19(LncRNA H19)与长链非编码 RNAHAND2-AS1(Ln- cRNA HAND2-AS1)表达水平，并分析其与病人胰岛素抵抗( IR)的相关性。方法将 2021年 1—12月在唐山市妇幼保健院建卡的子痫前期孕妇 105例作为子痫前期组，根据子痫前期孕妇严重程度分为轻度子痫前期组与重度子痫前期组，另选取同期孕周、年龄相符的健康孕妇 97例作为对照组，收集所有孕妇身体质量指数(BMI)、孕周、收缩压、舒张压、空腹胰岛素(FINS)、空腹血糖( FBG)等一般资料，并计算稳态模型的 IR指数( HOMA-IR)；实时荧光定量 PCR(qRT-PCR)法测定孕妇血清、胎盘组织中 LncRNA H19，LncRNA HAND2-AS1水平；比较对照组与子痫前期组、轻度子痫前期组与重度子痫前期组间血清、胎盘组织 LncRNA H19、LncRNA HAND2-AS1水平； Pearson法分析子痫前期孕妇血清、胎盘组织 LncRNA H19、LncRNA HAND2-AS1与 HOMA-IR的相关性。结果子痫前期组病人收缩压、舒张压高于对照组(P<0.05)且重度子痫前期病人收缩压、舒张压高于轻度子痫前期病人，孕周低于轻度子痫前期病人(P<0.05)；与对照组相比，子痫前期组孕，妇 24 h尿蛋白、 FBG［(5.84±0.97)mmol/L比(4.22±0.70)mmol/L］、FINS［(13.37±2.23)mU/L比( 7.52±1.25)mU/L］、HOMI-IR水平(3.47±0.57比 1.41±0.23)升高(P<0.05)，且重度子痫前期孕妇 24 h尿蛋白、 FBG［(6.90±1.15)mmol/L比( 5.24±0.85)mmol/L］、 FINS［(13.97±2.32)mU/L比( 13.05±2.17)mU/L］、 HOMI-IR水平( 4.27±0.71比 3.03±0.50)高于轻度子痫前期孕妇( P<0.05)；与对照组相比，子痫前期组孕妇血清、胎盘组织 Ln- cRNA H19(2.52±1.42比 1.01±0.15，3.75±0.62比 1.02±0.17)与 LncRNA HAND2-AS1水平( 1.98±0.33比 1.01±0.15，2.87±0.47比1.02±0.17)升高( P<0.05)且重度子痫前期孕妇血清、胎盘组织 LncRNA H19(2.84±0.47比 2.34±0.39，4.28±0.71比 3.45±0.57)与LncRNA HAND2-AS1水平(，2.59±0.43比 1.63±0.27，3.56±0.59比 2.49±0.41)高于轻度子痫前期孕妇( P<0.05)；子痫前期病人血清与胎盘组织间 LncRNA H19水平呈正相关( r=0.55，P<0.05)血清与胎盘组织间 LncRNA HAND2-AS1水平呈正相关性( r=0.65，P<0.05)。子痫前期孕妇血清、胎盘组织 LncRNA H19水别与 HOMI-IR呈正相关( r=0.53、0.59，P<0.05)；血清、胎盘组织 LncRNA HAND2-AS1水平分别与 HOMI-IR呈正相关( r=0.60、0.61，P<0.05)。结论子痫前期病人血清、胎盘组织 LncRNA 平分，H19、LncRNA HAND2-AS1高表达，二者与 IR密切相关，可能通过影响 IR参与子痫前期发生发展。 |
| 英文摘要: |
| Objective To detect the expression levels of long non-coding RNA H19 (LncRNA H19) and long non-coding RNA HAND2-AS1 (LncRNA HAND2-AS1) in placental tissue and serum of patients with preeclampsia, and to analyze their correlation with insulin resistance (IR).Methods One hundred and five pregnant women with preeclampsia who established cards in Tangshan Mater-nal and Child Health Hospital from January 2021 to December 2021 were taken as the preeclampsia group. According to the severity ofpreeclampsia, pregnant women were assigned into mild preeclampsia group and severe preeclampsia group. Another 97 healthy preg-nant women with the same gestational age and age were taken included in the control group. General information such as body mass in-dex (BMI), gestational age, systolic blood pressure, diastolic blood pressure, fasting insulin (FINS), and fasting blood glucose (FBG) ofall pregnant women were collected, and the IR index of the steady state model (HOMA-IR) was calculated; Real-time quantitative re- verse transcription PCR (qRT-PCR) method was used to determine the levels of LncRNA H19 and LncRNA HAND2-AS1 in maternal serum and placental tissue; the levels of LncRNA H19 and LncRNA HAND2-AS1 in serum and placental tissue were compared be-tween the control group, the preeclampsia group, the mild preeclampsia group and the severe preeclampsia group; Pearson's methodwas used to analyze the correlation between serum and placental tissue LncRNA H19, LncRNA HAND2-AS1 and HOMA-IR of pre- eclampsia pregnant women.Results The systolic blood pressure and diastolic blood pressure in the preeclampsia group were higher than those in the control group (P<0.05), and the systolic blood pressure and diastolic blood pressure in the patients with severe pre-eclampsia were higher than those in the patients with mild preeclampsia, and the gestational age was lower than that in the patients withmild preeclampsia (P<0.05); compared with the control group, the levels of 24-hour urine protein, FBG [(5.84±0.97) mmol/L vs. (4.22± 0.70) mmol/L], FINS [(13.37±2.23) mU/L vs. (7.52±1.25) mU/L] and HOMI-IR (3.47±0.57 vs. 1.41±0.23) of pregnant women in the pre- eclampsia group were increased (P<0.05), and the levels of 24-hour urine protein, FBG [(6.90±1.15) mmol/L vs. (5.24±0.85) mmol/L], FINS [(13.97±2.32) mU/L vs. (13.05±2.17) mU/L] and HOMI-IR (4.27±0.71 vs. 3.03±0.50) of pregnant women with severe preeclamp- sia were higher than those of pregnant women with mild preeclampsia (P<0.05); compared with the control group, the levels of LncRNA H19 (2.52±1.42 vs. 1.01±0.15 , 3.75±0.62 vs. 1.02±0.17) and LncRNA HAND2-AS1 (1.98±0.33 vs. 1.01±0.15 , 2.87±0.47 vs. 1.02± 0.17) in the serum and placental tissue of pregnant women in the preeclampsia group were increased (P<0.05), and the levels of Ln- cRNA H19 (2.84±0.47 vs. 2.34±0.39 , 4.28±0.71 vs. 3.45±0.57) and LncRNA HAND2-AS1 (2.59±0.43 vs. 1.63±0.27 , 3.56±0.59 vs. 2.49±0.41) in the serum and placental tissue of pregnant women with severe preeclampsia were higher in pregnant women with mildpreeclampsia (P<0.05); the level of LncRNA H19 between serum and placental tissue of patients with preeclampsia was positively cor- related (r=0.55, P<0.05), and the level of LncRNA HAND2-AS1 between serum and placental tissue was positively correlated (r=0.65, P<0.05). The level of LncRNA H19 in serum and placental tissue of pregnant women with preeclampsia was positively correlated withHOMI-IR respectively (r=0.53, 0.59, P<0.05); the level of LncRNA HAND2-AS1 in serum and placental tissue was positively correlat- ed with HOMI-IR (r=0.60, 0.61, P<0.05).Conclusion LncRNA H19 and LncRNA HAND2-AS1 are highly expressed in serum andplacental tissue of patients with preeclampsia, they are closely related to IR and may be involved in the occurrence and development of |
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