| 梁朝鑫,周安远,吕沛,等.富血小板血浆调控 SOX9对缓解 SD大鼠膝骨关节炎的影响[J].安徽医药,2024,28(2):229-234. |
| 富血小板血浆调控 SOX9对缓解 SD大鼠膝骨关节炎的影响 |
| Effect of platelet-rich plasma regulating SOX9 on alleviating osteoarthritis in SD rats |
| |
| DOI：10.3969/j.issn.1009-6469.2024.02.005 |
| 中文关键词: 骨关节炎，膝 富血小板血浆 SOX9激动剂 软骨细胞 脂多糖 |
| 英文关键词: Osteoarthritis,knee Platelet-rich plasma SOX9 agonist Chondrocytes LPS |
| 基金项目:广西壮族自治区自然科学基金项目( 2020GXNSFAA159162)；南宁市青秀区科技计划项目( 2020014) |
|
| 摘要点击次数: 391 |
| 全文下载次数: 226 |
| 中文摘要: |
| 目的探讨富血小板血浆细胞(PRP)与 Y染色体上的性别决定基因区域( SOX9)受体激动剂对缓解 SD大鼠膝骨关节炎综合征( KOA)的影响。方法实验自 2021年 4月至 2022年 3月，体外用脂多糖( LPS)诱导 SD大鼠关节软骨细胞炎症模型，不同浓度的 PRP与 SOX9激动剂干预经 LPS处理后的 SD大鼠软骨细胞，采用酶联免疫吸附测定( ELISA)与实时荧光定量聚合用酶链式反应( qPCR)检测相关疾病的 mRNA表达水平。体内注射实验通过在局部麻醉作用下假手术离断 SD大鼠的前交叉韧带而建立了 KOA模型，分为实验组(10只)、对照组( 10只)和假手术组大鼠( 10只)采用苏木精 -伊红( HE)染色、番红固绿染色及双甲苯胺蓝染色均显示无病理损伤，采用免疫组化染色检测软骨中基质金属蛋白酶，-13(MMP-13)和低聚蛋白多糖酶 -4(AD. AMTS-4)。结果体外实验， ELISA法结果显示，与正常组比较，在 LPS的影响下白细胞介素( IL)-1β(81.65±1.37比 58.3± 2.26)、 IL-6(73.3±1.75比 34.75±1.75)、肿瘤坏死因子 α(TNF-α)(22.76±0.37比 13.41±0.23)、诱导型一氧化氮合酶( iNOS)(1.06± 0.01比 0.84±0.02)浓度明显上升，加入不同浓度的 PRP后各炎症因子均明显下降( P<0.05)在加入 SOX9激动剂后各炎症因子下降更明显( 64.29±1.87、47.01±1.75、19.24±0.16、0.92±0.01)(均 P<0.01)； RT-PCR结果显示，，在 LPS的影响下 MMP-3、MMP-13、 ADAMTS-4、ADAMTS-5、IL-1β、IL-6、环氧化酶 -2抑制剂( COX-2)的 mRNA表达上升，而软骨蛋白聚糖抗体( ACAN)和 Ⅱ型多肽胶原酶( COL2A1)的表达量明显下降(均 P<0.05)在加入不同浓度的 PRP和 SOX9激动剂后在各指标均得到明显恢复，其中以 SOX9激动剂影响下最为明显(均 P<0.01)。体内病，理实验，通过膝关节软骨大体观检查和关节病理组织切片及染色等结果的显示，实验组关节软骨的局部病理软组织损伤及程度可较对照组明显减轻。结论富血小板血浆可能会通过调控 SOX9受体的表达，减少高聚蛋白多糖的丢失和抑制软骨细胞肥大因子及软骨细胞基质中的 Ⅱ型胶原质的诱导降解，并还能直接有效地抑制由于 LPS所致软骨的各种炎症因子蛋白的异常表达生成和诱导释放，从而可减少对软骨细胞的外源基质因子的诱导降解，达到缓解骨关节炎病程发展的目的。 |
| 英文摘要: |
| Objective To investigate the effects of Platelet-rich plasma (PRP) and SOX9 receptor agonists on alleviating knee osteoar. thritis (KOA) in SD rats.Methods From April 2021 to March 2022, the articular chondrocyte inflammation model was induced by li.popolysaccharide (LPS) in vitro, and the LPS-treated SD rat chondrocytes were treated with different concentrations of PRP and SOX9agonists, and the related diseases were detected quantitatively by ELISA and RT-PCR using real-time fluorescence quantitative genedetection mRNA expression levels of pathogenic genes.In vivo injection experiment, the anterior cruciate ligament of SD rats was sev.ered by sham operation under local anesthesia and KOA model was established. The rats in the experimental group (10 rats), controlgroup (10 rats) and sham operation group (10 rats) were divided into the experimental group (10 rats). No pathological damage wasshown by HE, safranine solid green staining and ditoluidine blue staining, and stromal gold in cartilage was detected by immunohisto.chemical staining It belongs to protease-13 (MMP-13) and polyproteoglycan enzyme-4 (ADAMTS-4).Results In vitro, the results of ELISA showed that the concentrations of IL-1β(81.65±1.37 vs. 58.3±2.26), IL-6(73.3±1.75 vs. 34.75±1.75), TNF-α(22.76±0.37 vs. 13.41±0.23) and iNOS(1.06±0.01 vs. 0.84±0.02) were significantly increased under the influence of LPS, and the inflammatory factorswere significantly decreased after adding different concentrations of PRP (P<0.05), and the inflammatory factors were significantly de.creased after adding SOX9 agonist(64.29±1.87,47.01±1.75,19.24±0.16,0.92±0.01) (all P<0.01). RT-PCR results showed that under the influence of LPS, the mRNA expressions of MMP-3, MMP-13, ADAMTS-4, ADAMTS-5, IL-1β, IL-6 and COX-2 were increased, while the expressions of ACAN and COLA2A1 were significantly decreased (all P<0.05). After the addition of different concentrations of PRPand SOX9 agonists, the indexes were significantly recovered, and the SOX9 agonist was the most significant (all P<0.01). In vivo patho.logical experiment, through the knee cartilage gross examination and joint pathological tissue section and staining results, the experi.mental results of the group of articular cartilage local pathological soft tissue injury and degree can be significantly reduced comparedwith the control group.Conclusion Platelet rich plasma may regulate the expression of SOX9 receptor through, to reduce the loss ofthe high poly proteoglycan and inhibition of cartilage cell hypertrophy factor and type Ⅱ collagen induced degradation of cartilage ma.trix, and can also directly effective inhibition caused by LPS cartilage inflammation of the various factor protein abnormal expression ofgeneration and induce the release,which can reduce exogenous of cartilage cells The induced degradation of matrix factors can alleviatethe progression of osteoarthritis. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
