| 吴凡,陈辉,张杞柳,等.液相色谱 -串联质谱法测定蓝芩口服液中 16种化学成分的含量[J].安徽医药,2024,28(2):251-255. |
| 液相色谱 -串联质谱法测定蓝芩口服液中 16种化学成分的含量 |
| Simultaneous determination of sixteen components in Lanqin oral solution by LC-MS/MS |
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| DOI：10.3969/j.issn.1009-6469.2024.02.009 |
| 中文关键词: 中草药 质量控制 高效液相色谱 -串联质谱法 蓝芩口服液 含量 |
| 英文关键词: Drugs,Chinese herbal Quality control LC-MS/MS Lanqin oral solution Content |
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| 中文摘要: |
| 目的建立同时测定蓝芩口服液中 16种化学成分含量的方法。方法采用液相色谱 -串联质谱法( LC-MS/MS)。 Fortis Xi C18柱( 100 mm×2.1 mm，1.7 μm)；流动相为 0.1%甲酸水溶液( A)-乙腈( B)；梯度洗脱；流速: 0.3 mL/min；进样量: 2 μL；柱温: 40 ℃；质谱采用电喷雾离子源( ESI)正负离子模式，多反应监测( MRM)方式进行测定。结果蓝芩口服液中汉黄芩苷、黄芩苷、异牡荆苷、栀子苷、巴马汀、黄柏碱、木兰花碱、小檗碱、鸟苷、汉黄芩素、黄芩素、腺苷、胞苷、表告依春、山栀苷和绿原酸等 16个成分浓度分别在所考察的浓度范围内与峰面积呈良好的线性关系( r>0.999 0)；精密度、稳定性、重复性 RSD均<2.5%，加样回收率( n=9)在 91.7%~108.3%范围内， RSD<3.5%。结论所建立的 LC-MS/MS方法简便、可靠、选择性好、灵敏度高，可用于蓝芩口服液的质量控制。 |
| 英文摘要: |
| Objective To develop a simple, rapid and sensitive method for the determination of sixteen compounds in Lanqin oral so. lution. Methods The method was LC-MS/MS. The analysis was performed on Fortis Xi C18(100 mm×2.1 mm, 1.7 μm) column with 0.1% formic acid-water and acetonitrile as mobile phase at a rate of 0.3 mL/min. The column temperature was set at 40 ℃ and the injec.tion volume was 2 μL. Ectrospray ionization(ESI) interface and multiple reaction monitoring(MRM) were used.Results All calibration curves of sixteen components(wogonoside, baicalin, isovitexin, geniposide, palmatine, phellodendrine, magnoflorine, berberine, guano.sine, wogonin, baicalein, adenosine, cytidine, epigoitrin, shanzhiside, chlorogenic acid) were linear (r>0.999 0) over the tested ranges. The repeatability, precision and stability with RSD<2.5%. The average recoveries (n=9) ranged from 91.7% to 108.3% with RSD<3.5%. The results of the sixteen compounds of samples all met the requirements of the national standards, but the content of the sixteencomponents between batches varied greatly.Conclution The established LC-MS/MS method is convenient, reliable, with good selec. tivity and high sensitivity, which can be used for the quality control of Lanqin oral solution. |
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