| 曹志军,张智伟,李志国,等.长链非编码 RNA DNAJC3-AS1、微 RNA-214-3p在结肠癌组织、结肠腺瘤性息肉中的表达水平及临床意义[J].安徽医药,2024,28(2):366-370. |
| 长链非编码 RNA DNAJC3-AS1、微 RNA-214-3p在结肠癌组织、结肠腺瘤性息肉中的表达水平及临床意义 |
| Expression and clinical significance of long non-coding RNA DNAJC3-AS1 and microRNA-214-3p in colon adenomatous polyps and colon cancer tissues |
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| DOI：10.3969/j.issn.1009-6469.2024.02.034 |
| 中文关键词: 长链非编码 RNA DNAJC3-AS1 结肠肿瘤 微 RNA-214-3p 结肠腺瘤性息肉 |
| 英文关键词: Long non-coding RNA DNAJC3-AS1 Colonic neoplasms MicroRNA-214-3p Colon adenomatous polyps |
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| 中文摘要: |
| 目的探讨长链非编码 RNA(lncRNA)DNAJC3-AS1、微 RNA-214-3p(miR-214-3p)在结肠癌组织、结肠腺瘤性息肉中的表达水平及临床意义。方法 lnCAR数据库检索结肠癌组织、正常结肠组织中 LncRNA DNAJC3-AS1表达情况；选取 2016年 5月至 2019年 1月北京市丰台中西医结合医院收治的 86例结肠癌病人、 53例结肠腺瘤性息肉病人为研究对象，收集结肠癌病人的结肠癌组织、对应癌旁组织及结肠腺瘤性息肉病人的结肠腺瘤性息肉，实时荧光定量 PCR(qRT-PCR)法测定组织中 lncRNA DNAJC3-AS1、miR-214-3p表达水平；分析结肠癌组织 lncRNA DNAJC3-AS1、miR-214-3p表达水平与结肠癌病人临床病理特征、预后关系；分析结肠癌组织中 lncRNA DNAJC3-AS1表达水平与 miR-214-3p相关性，生物信息学预测 lncRNA DNAJC3-AS1与 miR-214-3p的关系。结果 lnCAR数据库分析显示，结肠癌组织 lncRNA DNAJC3-AS1表达水平( 4.40±0.49)高于正常结肠组织( 4.00±0.36)(P<0.05)。 qRT-PCR检测显示，与结肠腺瘤性息肉( 1.71±0.57、0.67±0.22)相比，结肠癌组织 lncRNA DNAJC3-AS1表达水平( 2.63±0.88)升高( P<0.05)miR-214-3p表达水平( 0.31±0.10)降低( P<0.05)；与癌旁组织( 1.04±0.35、1.01±0.34)相比，结肠癌组织 lncRNA DNAJC3-AS1表达，水平升高( P<0.05)， miR-214-3p表达水平降低( P<0.05)。结肠癌组织 lncRNA DNAJC3-AS1、miR-214-3p表达水平在不同分化程度、淋巴结转移、血管浸润、 TNM分期方面，分布差异有统计学意义( P<0.05)；结肠癌组织中 lncRNA DNAJC3-AS1表达水平与 miR-214-3p呈负相关( r=.0.58，P<0.05)，且 lncRNA DNAJC3-AS1与 miR-214-3p存在结合位点； lncRNA DNAJC3-AS1高表达组 29.75个月、 miR-214-3p低表达组 30.16个月结肠癌病人累积生存率低于 ln. cRNA DNAJC3-AS1低表达组 34.69个月、 miR-214-3p高表达组 34.37个月( P<0.05)。结论癌旁组织、结肠腺瘤性息肉、结肠癌组织中 lncRNA DNAJC3-AS1表达依次升高， miR-214-3p表达依次降低，且结肠癌组织中 lncRNA DNAJC3-AS1表达水平与 miR-214-3p、临床病理特征、预后有关。 |
| 英文摘要: |
| Objective To investigate the expression levels and clinical significance of long non-coding RNA (lncRNA) DNAJC3-AS1 and microRNA-214-3p (miR-214-3p) in colon cancer (CC) tissue and colon adenomatous polyps.Methods The lnCAR database was retrieved to search the expression of LncRNA DNAJC3-AS1 in CC tissue and normal colon tissue. A total of 86 patients with CC and 53patients with colonic adenomatous polyps admitted to Fengtai Hospital of Integrated Traditional Chinese and Western Medicine fromMay 2016 to January 2019 were taken as the research objects. The CC tissue of CC patients, the corresponding paracancerous tissueand colonic adenomatous polyps of patients with colonic adenomatous polyps were collected. The expression levels of lncRNA DNAJC3-AS1 and miR-214-3p in tissues were determined by real-time quantitative PCR (qRT-PCR) method. The relationship between the ex. pression levels of lncRNA DNAJC3-AS1 and miR-214-3p in CC tissues and the clinicopathological characteristics and prognosis of CCpatients was analyzed. The correlation between the expression level of lncRNA DNAJC3-AS1 and miR-214-3p in CC tissues was ana. lyzed, and the relationship between lncRNA DNAJC3-AS1 and miR-214-3p was predicted by bioinformatics.Results lnCAR database analysis showed that lncRNA DNAJC3-AS1 expression level in CC tissues (4.40±0.49) was higher than that in normal colon tissues (4.00±0.36) (P<0.05). qRT-PCR showed that lncRNA DNAJC3-AS1 expression level in CC tissues was higher (2.63±0.88) than that in colorectal adenomatous polyps (1.71±0.57, 0.67±0.22) (P<0.05). The expression level of miR-214-3p was decreased (0.31±0.10) (P< 0.05). Compared with para-cancerous tissues (1.04±0.35, 1.01±0.34), the expression level of lncRNA DNAJC3-AS1 in CC tissues was increased (P<0.05), and the expression level of miR-214-3p was decreased (P<0.05). The expression levels of lncRNA DNAJC3-AS1 and miR-214-3p in CC tissues were significantly different in different degrees of differentiation, lymph node metastasis, vascular infil.tration and TNM stage (P<0.05). The expression level of lncRNA DNAJC3-AS1 in CC tissues was negatively correlated with miR-214-3p (r=.0.58, P<0.05), and there was a binding site between lncRNA DNAJC3-AS1 and miR-214-3p. The cumulative survival rate of CC patients was 29.75 (months) in the lncRNA DNAJC3-AS1 high expression group, 30.16 (months) in the miR-214-3p low expression group, 34.69 (months) in DNAJC3-AS1 low expression group, and 34.37 (month) in miR-214-3p high expression group (P<0.05).Con. clusion The expressions of lncRNA DNAJC3-AS1 in paracancerous tissue, colonic adenomatous polyps and CC tissue increase suc. cessively, while the expression of miR-214-3p decreases successively. LncRNA DNAJC3-AS1 is related to miR-214-3p, clinicopatho. logical features, and prognosis. |
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