| 张婉君,孔中楠,马永平,等.固定正畸矫治 80例病人唾液基质金属蛋白酶 8和基质金属蛋白酶 9的水平变化[J].安徽医药,2024,28(4):746-750. |
| 固定正畸矫治 80例病人唾液基质金属蛋白酶 8和基质金属蛋白酶 9的水平变化 |
| Changes of saliva MMP-8 and MMP-9 levels in fixed orthodontic patients |
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| DOI：10.3969/j.issn.1009-6469.2024.04.023 |
| 中文关键词: 牙龈炎 正畸矫正器 基质金属蛋白酶 8 基质金属蛋白酶 9 牙菌斑指数 |
| 英文关键词: Gingivitis Orthodontic appliances Matrix metalloproteinases 8(MMP-8) Matrix metalloproteinases 9(MMP-9) Dental plaqueindex |
| 基金项目:河北省科技计划项目( 212777154) |
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| 中文摘要: |
| 目的探究固定正畸矫治病人唾液基质金属蛋白酶 8(MMP-8)、基质金属蛋白酶 9(MMP-9)水平的变化及临床意义。方法选取 2019年 6月至 2021年 12月于保定市第二医院接受固定正畸矫治成年病人 80例及健康受试者 40例为研究对象。对病人固定矫治前( T1)、固定矫治 1周( T2)、固定矫治 1个月( T3)及健康受试者探诊出血( BOP)、菌斑指数( PLI)进行检测；收集健康受试者及病人 T1、T2、T3时期唾液，采用 ELISA检测唾液中 MMP-8、MMP-9水平；流式细胞术对唾液白细胞含量进行检测；分别对 MMP-8、MMP-9与牙龈 BOP相关性进行分析；分离唾液白细胞，检测白细胞 MMP-8、MMP-9表达水平变化。结果 T2、T3时病人 BOP［( 21.36±8.79)%、(13.06±5.80)%］、 PLI［( 2.53±0.43)分、(1.89±0.39)分］及唾液中 MMP-8［( 0.43±0.11)μg/L、(0.32±0.10)μg/L］、 MMP-9［( 3.64±0.76)μg/L、(2.02±0.50)μg/L］、白细胞水平［( 17 893.71±505.49)个、(8 532.18±421.89)个］，显著高于 T1水平［(5.05±2.11)%、(0.71±0.25)分、(0.16±0.08)μg/L、(0.25±0.13)μg/L、(2 308.66±178.04)个， P<0.05］；唾液 MMP-8、 MMP-9水平与牙龈 BOP、PLI呈正相关；进一步发现 T2、T3时白细胞 MMP-8、MMP-9表达水平显著高于 T1水平( P<0.05)。受试者操作特征( ROC)曲线显示唾液 MMP-8及 MMP-9水平能较好预测牙龈炎程度。结论正畸病人唾液 MMP-8、MMP-9水平与牙龈炎病情呈正相关，唾液 MMP-8、MMP-9可作为评估正畸病人牙龈炎的潜在生物标志物。 |
| 英文摘要: |
| Objective To explore the changes and clinical significance of saliva matrix metalloproteinases 8 (MMP-8) and matrix me? talloproteinases 9 (MMP-9) levels in fixed orthodontic patients.Methods A total of 80 adult patients and 40 healthy subjects who re?ceived fixed corrective treatment in The Second Hospital of Baoding City from June 2019 to December 2021 were selected as the studysubjects. The bleeding on probing (BOP) and plaque index (PLI) of patients before fixed orthodontic treatment (T1), 1 week after treat?ment (T2), 1 month after treatment (T3) and healthy subjects were detected. Saliva of healthy subjects and patients at T1, T2 and T3were collected, and the levels of MMP-8 and MMP-9 in saliva were detected by ELISA. Flow cytometry was performed to detect leuko?cyte concentration in saliva. The correlations between MMP-8, MMP-9 and gingival BOP were analyzed, respectively. White blood cells in saliva were isolated, and the expression levels of MMP-8 and MMP-9 in leukocytes were detected.Results At T2 and T3, the levels of BOP [(21.36±8.79) %, (13.06±5.80) %], PLI [(2.53±0.43) points, (1.89±0.39) points] and saliva MMP-8 [(0.43±0.11) μg/L, (0.32± 0.10) μg/L], MMP-9 [(3.64±0.76) μg/L, (2.02±0.50) μg/L], leukocyte counts [(17 893.71±505.49), (8 532.18±421.89)] were significant?ly higher than those at T1 [(5.05±2.11) %, (0.71±0.25) points, (0.16±0.08) μg/L, (0.25±0.13) μg/L, (2 308.66±178.04), P<0.05]. Sali? vary MMP-8 and MMP-9 levels were positively correlated with gingival BOP and PLI. It was further found that the expression levels of MMP-8 and MMP-9 in leukocytes at T2 and T3 were significantly higher than those at T1 (P<0.05). Receiver operating characteristic (ROC) curve showed that salivary MMP-8 and MMP-9 levels could better predict the degree of gingivitis.Conclusion The levels of MMP-8 and MMP-9 in saliva are positively correlated with the condition of gingivitis in orthodontic patients, and MMP-8, MMP-9 in sa? liva can be used as potential biomarkers to evaluate the gingivitis in orthodontic patients. |
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