| 杨睿,李占江,陈腾飞.YTH结构域家族蛋白 2和叉头蛋白转录因子 3在肺腺癌中的表达关系研究[J].安徽医药,2024,28(4):764-767. |
| YTH结构域家族蛋白 2和叉头蛋白转录因子 3在肺腺癌中的表达关系研究 |
| Expression relationship of YTHDF2 and FOXO3 in lung adenocarcinoma |
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| DOI：10.3969/j.issn.1009-6469.2024.04.027 |
| 中文关键词: 癌，非小细胞肺 肺腺癌 YTH结构域家族蛋白 2 叉头蛋白转录因子 3 临床病理特征 累积生存率 |
| 英文关键词: Carcinoma,non-small-cell lung Lung adenocarcinoma YTH domain family protein 2 Forkhead transcription factor 3 Clinicopathological characteristics Cumulative survival rate |
| 基金项目:河南省医学科技攻关计划联合共建项目( LHGJ20190209) |
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| 中文摘要: |
| 目的分析 YTH结构域家族蛋白 2(YTHDF2)及叉头蛋白转录因子 3(FOXO3)在肺腺癌病人中的表达及与预后的关系。方法收集 2020年 1月至 2021年 5月在郑州大学第一附属医院行手术治疗的肺腺癌病人癌组织及对应的癌旁组织(距离癌组织 5 cm以上) 80对，收集病人临床病理资料；利用癌症基因组图谱( TCGA)数据库查询 YTHDF2、FOXO3基因在肺腺癌中的表达水平；采用免疫组织化学法检测 YTHDF2、FOXO3蛋白在肺腺癌组织中的表达情况；分析 YTHDF2、FOXO3蛋白水平与肺腺癌病人临床病理资料的关系；分析肺腺癌中 YTHDF2与 FOXO3基因表达水平的相关性；对病人进行为期 3年的随访，分析病人 3年累积生存率。结果 YTHDF2、FOXO3蛋白在肺腺癌组织中的高表达率分别为 34.00%、26.00%，明显低于癌旁正常组织中 79.48%、61.54%(P<0.05)。 YTHDF2蛋白表达情况与肺腺癌病人年龄、淋巴结转移和分化程度相关( P<0.05)；与 TNM分期、性别无关( P>0.05)； FOXO3蛋白表达情况与肺腺癌病人性别和分化程度相关(P<0.05)；肺腺癌组织中 YTHDF2蛋白高表达组和低表达组病人 3年累积生存率分别为 53.30%、14.00%，经 log-rank比较，差异有统计学意义( P<0.05)； FOXO3蛋白高表达组和低表达组病人 3年累积生存率分别为 64.50%、12.20%，经 Log-Rank比较，差异有统计学意义( P<0.05)。结论 YTHDF2、 FOXO3在肺腺癌组织中均低表达，与病人肿瘤分化程度及 3年累积生存率有关，有望成为评估肺腺癌病人预后的生物标志物。 |
| 英文摘要: |
| Objective To analyze the expressions of YTH domain family protein 2 (YTHDF2) and forkhead transcription factor 3(FOXO3) in lung adenocarcinoma and their relationship with the prognosis of patients.Methods Eighty pairs of cancerous tissues and their corresponding normal adjacent tissues (more than 5 cm from the cancerous tissues) were collected from patients with lung adeno?carcinoma who underwent surgical treatment in The First Affiliated Hospital of Zhengzhou University from January 2020 to May 2021.The clinical pathological data of patients were collected. The Cancer Genome Atlas (TCGA) database was used to query the expressionlevels of YTHDF2 and FOXO3 genes in lung adenocarcinoma. Immunohistochemistry was applied to detect the expressions ofYTHDF2 and FOXO3 proteins in lung adenocarcinoma tissues. The relationship between YTHDF2 and FOXO3 protein levels and clini?copathological data of patients with lung adenocarcinoma was analyzed, and the correlation between YTHDF2 and FOXO3 gene expres?sion levels in lung adenocarcinoma was analyzed. The patients were followed up for 3 years, and the 3-year cumulative survival rate of patients was analyzed. Results The high expression rates of YTHDF2 and FOXO3 proteins in lung adenocarcinoma tissues were34.00% and 26.00%, which were significantly lower than 79.48% and 61.54% in normal adjacent tissues (P<0.05). The expression ofYTHDF2 protein was correlated with the age, lymph node metastasis and differentiation degree of patients with lung adenocarcinoma (P< 0.05), while was not correlated with TNM staging and gender (P>0.05). The expression of FOXO3 protein was correlated with the gen?der and degree of differentiation of patients with lung adenocarcinoma (P<0.05). The 3-year cumulative survival rates of patients in the high-expressed YTHDF2 protein group and low-expressed YTHDF2 protein group were 53.30% and 14.00%, respectively, and Log-Rank comparison results showed that the difference was statistically significant (P<0.05); the 3-year cumulative survival rates in the high-expressed FOXO3 protein group and low-expressed FOXO3 protein group were 64.50% and 12.20%, respectively, and Log-Rank comparison results showed that the difference was statistically significant (P<0.05).Conclusions YTHDF2 and FOXO3 are both lowlyexpressed in lung adenocarcinoma tissues, which are related to the degree of tumor differentiation and the 3-year cumulative survivalrate of patients. They are expected to be biomarkers for evaluating the prognosis of patients with lung adenocarcinoma. |
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