| 刘晓玲,梁晓东,崔杨,等.反相高效液相色谱法测定曲咪新乳膏中醋酸曲安奈德和硝酸咪康唑的含量[J].安徽医药,2019,23(3):477-479. |
| 反相高效液相色谱法测定曲咪新乳膏中醋酸曲安奈德和硝酸咪康唑的含量 |
| Determination of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide and miconazole nitrate and neomycin sulfate cream by reversed-phase high performance liquid chromatography |
| 投稿时间：2017-08-08 |
| DOI： |
| 中文关键词: 此方法可同时测定曲咪新乳膏中两组分的含量,方法简便,准确,快速。 关键词:曲咪新乳膏 醋酸曲安奈德 硝酸咪康唑 反相高效液相色谱 |
| 英文关键词: Triamcinolone acetonide and miconazole nitrate and neomycin sulfate cream Triamcinolone acetonide acetate Miconazole nitrate RP-HPLC |
| 基金项目: |
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| 摘要点击次数: 3815 |
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| 中文摘要: |
| 目的 探讨测定曲咪新乳膏中醋酸曲安奈德和硝酸咪康唑含量的方法。 方法 采用反相高效液相色谱法。色谱柱为Agilent SB-C18(150 mm×4.6 mm,5.0 μm)；以A甲醇∶0.05%乙酸铵(85∶15),B甲醇∶0.05%乙酸铵(75∶25)为流动相,采用梯度洗脱；流速1.0 mL/min；柱温30 ℃；检测波长240 nm,进样量:20 μL。 结果 醋酸曲安奈德在0.01~0.06 mg/mL范围内线性关系良好(r=1.000 0,n=5),平均回收率为100.52%,RSD=0.62%；硝酸咪康唑在0.1~0.6 mg/mL范围内线性关系良好(r=0.999 6,n=5),平均回收率为99.93%,RSD=0.86%。 |
| 英文摘要: |
| Objective To establish a RP-HPLC method to determinate the contents of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide and miconazole nitrate and neomycin sulfate cream. Methods Using Agilent SB-C18(150 mm×4.6 mm,5.0 μm)column;Mobile phase Awas methyl alcohol and 0.05% ammonium acetate in (85∶15),Mobile phase Bwas methyl alcohol and 0.05% ammonium acetate in (75∶25),gradient elution;Velocity:1.0 mL/min;The column temperature was 30 ℃.The UV detection wavelength was 240 nm and the inject volume was 20 μL. Result The linear hypotaxis of triamcinolone acetonide was good in the scope of 0.01-0.06 mg/mL (r=1.000 0,n=5).The average recycle ratio was 100.52% (RSD=0.62%).The linear hypotaxis of Miconazole Nitrate was good in the scope of 0.1-0.6 mg/mL (r=0.999 6,n=5).The average recycle ratio was 99.93% (RSD=0.86%). Conclusion This method can simultaneously determine the contents of the two components in triamcinolone acetonide and miconazole nitrate and neomycin sulfate cream.The method is simple,accurate and fast. |
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