| 张雅馨,苏杰,黄帅,等.丁苯酞对兔视网膜缺血再灌注损伤中细胞凋亡的影响[J].安徽医药,2019,23(7):1302-1305. |
| 丁苯酞对兔视网膜缺血再灌注损伤中细胞凋亡的影响 |
| Effect of Dl-3n-butylphthalide on retinal cell apoptosis in rabbits with ischemia-reperfusion injury |
| 投稿时间：2017-12-30 |
| DOI： |
| 中文关键词: 丁苯酞 视网膜缺血再灌注 基因,P53 基因,Bcl-2 凋亡 |
| 英文关键词: Dl-3n-Butylphthalide Retinal ischemia-reperfusion injury Genes,P53 Genes,Bcl-2 Apoptosis |
| 基金项目: |
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| 摘要点击次数: 1957 |
| 全文下载次数: 541 |
| 中文摘要: |
| 目的 观察丁苯酞对兔视网膜缺血再灌注损伤(RIRI)后凋亡因子P53/Bcl-2表达的影响。方法 选取新西兰白兔80只(均为雄性),采用随机数字表法分为对照组10只,RIRI组和给药组各35只,其中对照组再分为6 h、24 h两个亚组,RIRI组和给药组均再分为再灌注后1 h、6 h、12 h、24 h、48 h、72 h、7 d等7个亚组。制作兔RIRI模型的方法采用高眼压灌注法,HE染色法观察视网膜组织形态变化,免疫组化法检测P53及Bcl-2的表达,TUNEL法检测神经节细胞的凋亡。结果 P53、Bcl-2及TUNEL凋亡细胞均在对照组检测到极少的表达,在RIRI组组内不同时间点的表达均为先增多后减少的趋势,24 h时达到最高峰,分别为(22.60±1.14)(P53、24 h)、(28.00±1.87)(Bcl-2,4 h)、(27.20±0.83)(凋亡,24 h),差异有统计学意义(均P＜0.01)；给药组最高峰出现在24 h,分别为(13.40±0.54)(P53、24 h)、(34.60±1.67)(Bcl-2,24 h)、(19.40±2.30)(凋亡,24 h),差异有统计学意义(均P＜0.01)。组间比较P53及TUNEL凋亡细胞的表达各个时间点均较RIRI组明显减少(均P＜0.05),Bcl-2蛋白的表达较RIRI组增多(P＜0.01)。结论 RIRI后行丁苯酞药物治疗可抑制视网膜神经节细胞P53的表达,增强Bcl-2的表达,减少神经节细胞的凋亡,对RIRI有一定的保护作用。 |
| 英文摘要: |
| Objective To observe the effect of butylphthalide on the expression of apoptotic factor P53/Bcl-2 after retinal ischemia-reperfusion injury in rabbits.Methods A total of 80 healthy New-Zealand white rabbits were randomly divided into control group (n=10),RIRI group (n=35) and drug group (n=35).The control group was subdivided into 2 subgroups:6 h,24 h.The RIRI group and drug group was subdivided into 7 subgroups:1 h,6 h,12 h,24 h,48 h,72 h and 7 d after reperfusion.The rabbit model of retinal ischemia-reperfusion injury was established by high intraocular pressure perfusion.To observe morphology and degree of injury,detecting the expression of P53 and Bcl-2 in the retina by immunohistochemical method and detecting apoptosis of retinal ganglion cell by TUNEL was performed.Results P53,Bcl-2 and TUNEL apoptotic cells were rarely expressed in the control group,and increased first and then decreased in 1,6,12,4,48,2 h and 7 d after retinal ischemia reperfusion of RIRI group.The peak respectively appeared at 24 h,which were (22.60±1.14)(P53,4 h),(28.00±1.87)(Bcl-2,4 h),(27.20±0.83)(apoptotic,24 h),with statistical significance (all P＜0.01).In drug group,the peak respectively appeared in 24 h,which were (13.40±0.54)(P53,4 h),(34.60±1.67)(Bcl-2,4 h),(19.40±2.30)(apoptotic,24 h),and the difference was statistically significant (all P＜0.01).The expressions of P53 and TUNEL apoptotic cells in each group were significantly lower than those in the RIRI group at each time point (P＜0.05),and Bcl-2 protein increased in RIRI group (P＜0.01).Conclusion Butylphthalide treatment can inhibit the P53 ganglion cells,enhance the expression of Bcl-2,reduce the apoptosis of retinal ganglion cells after retinal ischemia-reperfusion injury,which has a protective effect on retinal ischemia-reperfusion injury. |
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