| 赵谦,白艳霞,张少强,等.N?myc下游调控基因 2蛋白在喉鳞状细胞癌中的表达及对人喉表皮样癌细胞生物学特性的影响[J].安徽医药,2020,24(10):1933-1937. |
| N?myc下游调控基因 2蛋白在喉鳞状细胞癌中的表达及对人喉表皮样癌细胞生物学特性的影响 |
| Expression of NDRG2 in laryngeal squamous cell carcinoma and its effects on cellbiological character of Hep?2 cells |
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| DOI：10.3969/j.issn.1009?6469.2020.10.005 |
| 中文关键词: 喉肿瘤 癌，鳞状细胞 基因， myc 基因表达调控，肿瘤 人喉表皮样癌细胞 转移 侵袭 |
| 英文关键词: Laryngeal neoplasms Carcinoma,squamous cell Genes,myc Gene expression regulation,neoplastic Hep?2 cells Migration Invasion |
| 基金项目:陕西省自然科学基础研究计划面上项目(2017JM8072) |
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| 中文摘要: |
| 目的检测 N?myc下游调控基因 2(N?myc downstream regulated gene 2，NDRG2)在喉鳞状细胞癌组织中的表达及其人类上皮细胞 2型((Human Epithelial cells of type 2)对人喉表皮样癌细胞(Hep?2细胞)侵袭的影响。方法收集西安交通大学第一附属医院 2014年 1月 1日至 2017年 12月 31日接受喉部分切除术的病人，共计 41例喉鳞状细胞癌组织及癌旁组织。采用免疫组织化学检测喉鳞状细胞癌组织中 NDRG2的表达， χ2检验分析不同临床病理特征分组中 NDRG2阳性率的差异；实时定量聚合酶链式反应及蛋白质印迹法检测喉鳞状细胞癌 Hep?2细胞中 NDRG2的表达水平； Transwell检测 NDRG2对 Hep?2细胞侵袭及转移的影响。结果 41例喉鳞状细胞癌组织中 NDRG2的阳性率明显低于癌旁组织，(χ2=30.781，P＜0.05)TNM III期和 IV期及淋巴结转移的喉鳞状细胞癌病人中 NDRG2的阳性率低于 TNM I期和 II期及未发生转移的病人(TNM I和 TNM II NDRG2阳性率: 37.500%，TNM III和 IV NDRG2阳性率: 5.882%，χ2=5.394，P＜0.05；无淋巴结转移病人 NDRG2阳性率:55.556%，有淋巴结转移病人 NDRG2阳性率: 15.625，χ2=6.073，P＜0.05)。此外， Hep?2细胞中 NDRG2的表达也明显低于正常鼻咽上皮 NP?69细胞［Hep?2(1.193±0.053)，NP?69(0.238±0.072)，t=3.536，P＜0.05］。上调 Hep?2细胞中 NDRG2的表达后，转移［Lv?control(141.328±23.424)个， Lv?NDRG2(54.925±16.825)个， t=4.413，P＜0.05］及侵袭［Lv?control(71.782±18.675)个， Lv? NDRG2(25.931±9.729)个， t=3.757，P＜0.05］细胞数目显著降低。结论 NDRG2在喉鳞状细胞癌组织及细胞中低表达，并可作为抑癌基因抑制 Hep?2细胞增殖、侵袭及转移。 |
| 英文摘要: |
| Objective To investigate the expression of N?myc downstream regulated gene 2(NDRG2)in tissues of laryngeal squa? mous cell carcinoma(LSCC)and its effect on human epithelial cells of type 2(Hep?2)invasion.Methods A total of 41 cases of la? ryngeal squamous carcinoma tissues and adjacent tissues who underwent partial laryngectomy in the First Affiliated Hospital of Xi’ an Jiaotong University from January 1，2014 to December 31，2017were collected.Immunohistochemestry(IHC)was performed to de? tect the expression of NDRG2 in tissues of LSCC，chi?square analysis was performed to analyze the difference of NDRG2 positiverate in different clinicopathologicalfeature groups.Quantitative polymerase chain reaction(QPCR)and western blot(WB)were per? formed to detect the expression of NDRG2 in Hep?2 cells，and Transwell assays were performed to detect the effects of NDRG2 on cell migration and invasion of Hep?2 cells.Results The positive rates of NDRG2 in tissues of 41 cases of LSCC were lower than that in adjacent tissues(χ2=30.781，P＜0.05).Compared to LSCC patients at TNM I and TNM II stage or without lymph node metas? tasis，the positive rates of NDRG2 in LSCC patients at TNM III and TNM IV stage or with lymph node metastasis were significantlydown?regulated(NDRG2 positive ratesinTNM I andTNM II:37.500%，NDRG2 positive ratesinTNM III andTNM IV:5.882%，χ2=5.394，P＜0.05；NDRG2 positive ratesinpatientswithlymphnodemetastasis:55.556%，NDRG2 positive ratesinpatientswithoutlym? phnodemetastasis:15.625%，χ2=6.073，P＜0.05).Furthermore，the expression of NDRG2 was also lower in Hep?2 cells than that in normal nasopharyngeal epithelial NP?69 cells［Hep?2(1.193±0.053)NP?69(0.238±0.072)，t=3.536，P＜0.05］.After upregulating the expression of NDRG2 in Hep?2 cells， control(141.328±23.424)，Lv?NDRG2(54.925±16.825)，t=thenumbersofmigratory［Lv?4.413，P＜0.05］and invasive［Lv?control(71.782±18.675)，Lv?NDRG2(25.931±9.729)，t=3.757，P＜0.05］cells were significantly reduced.Conclusion NDRG2 is down?regulated in tissues and cells of LSCC.NDRG2 functions as tumor suppressor to inhibit cellmigration and invasion of Hep?2 cells. |
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