| 周湘,杜宁,刘昕.肿瘤浸润性树突状细胞在口腔部鳞状细胞癌组织中的表达及价值[J].安徽医药,2020,24(10):2006-2009. |
| 肿瘤浸润性树突状细胞在口腔部鳞状细胞癌组织中的表达及价值 |
| Expression and value of tumor infiltrating dendritic cells in oral squamous cell carcinoma |
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| DOI：10.3969/j.issn.1009?6469.2020.10.023 |
| 中文关键词: 肿瘤，鳞状细胞 口腔肿瘤 免疫组织化学 胞间黏附分子 1 基因， MHCⅡ类 肿瘤浸润性树突状细胞 |
| 英文关键词: Neoplasms,squamous cell Mouth neoplasms Immunohistochemistry Intercellular adhesion molecule ? 1 Genes,MHC class Ⅱ Neoplastic infiltrating dendritic cells |
| 基金项目:河北省医学科学研究重点课题(20190131) |
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| 中文摘要: |
| 目的探讨口腔部鳞状细胞癌病人癌组织中肿瘤浸润性树突状细胞的表达及其价值。方法选择 2017年 6月至 2018年 6月河北省儿童医院收治的 50例口腔部鳞状细胞癌病人作为观察组，选取 50例同时期来该院进行治疗的口腔良性肿物病人作为本次研究的对照组。取两组病人的肿物组织标本进行免疫组织化学染色法分析肿瘤浸润性树突状细胞密度，采用流式细胞仪计数肿瘤浸润性树突状细胞的人主要组织相容性复合体 Ⅱ类(MHC?Ⅱ)阳性细胞比例和白细胞抗原 54(CD54)阳性细胞比例。结果观察组肿瘤肿瘤淋巴结转移分类(TNM)分期为 Ⅲ期的肿瘤浸润性树突状细胞密度、 MHC?Ⅱ阳性肿瘤浸润性树突状细胞比例及 CD54阳性肿瘤浸润性树突状细胞比例均明显低于 Ⅰ/Ⅱ期［(7.61±1.43)比(8.65±1.42)，P=0.014；(5.31±1.80)%比(7.46±1.95)%，P=0.000；(6.62±2.11)比(8.21±1.86)%，P=0.008］；肿瘤组织的最大直径 ≥5 cm的肿瘤浸润性树突状细胞密度、 MHC?Ⅱ阳性肿瘤浸润性树突状细胞比例及 CD54阳性肿瘤浸润性树突状细胞比例均明显低于肿瘤组织直径＜ 5 cm的病人［(7.14±1.85)比(9.07±2.11)P=0.001；(5.42±1.84)比(7.73±1.85)，P=0.000；(6.34±2.06)比(8.47±1.91)，P=0.000］；观察组比均差异无统计学意义(P＞0.05)；观察组肿瘤浸润性树突状细胞密度明显低于对照组肿瘤分化程度不同的病人上述指标相,［(8.27±2.11)比(9.68±2.38)P=0.002］MHC?Ⅱ阳性肿瘤浸润性树突状细胞比例明显低于对照组［(6.67±2.20)比(9.79±2.58)， P=0.000］，CD54阳性肿瘤浸润，性树突状细，胞比例明显低于对照组［(7.46±2.31)比(9.83±2.54)P=0.000］。结论口腔部鳞状细胞癌病人癌组织内肿瘤浸润性树突状细胞的密度下降、 MHC?Ⅱ阳性及 CD54阳性的细胞比例降，低，且随着 TNM分期及肿瘤直径的增大，肿瘤浸润性树突状细胞的表达水平更低。 |
| 英文摘要: |
| Objective To investigate the expression and value of invasive dendritic cells in oral squamous cell carcinoma(SCC). Method Fifty patients with oral squamous cell carcinoma admitted to Hebei Provincial Children′s Hospital from June 2017 to June2018 were selected as the observation group，and 50 patients with benign oral masses treated in our hospital during the same peri?od were selected as the control group.The neoplasm tissue samples of patients in the two groups were taken for immunohistochemi?cal staining to analyze the density of tumor infiltrating dendritic cell，and flow cytometry was used for counting of tumor infiltrating dendritic cells MHC?Ⅱ positive cells and CD54 positive cells.Result In the observation group，the density of infiltrating dendritic cells，the proportion of MHC?Ⅱ?positive tumor infiltrating dendritic cells，and the proportion of CD54?positive tumor infiltrating den? dritic cells in tumors with tumor lymph node metastasis classification(TNM)stage III were significantly lower than those with Ⅰ/ Ⅱ stage［(7.61±1.43)vs.(8.65±1.42)，P=0.014，(5.31±1.80)% vs.(7.46±1.95)%，P=0.000，and(6.62±2.11)% vs.(8.21±1.86)%，P =0.008］； the tumor infiltrating dendritic cell density，MHC?Ⅱ positive cell rate and CD54 positive cells in tumor with the maxi?mum diameter of ≥ 5 cm were significantly lower than those of patients with tumor diameter＜5 cm［(7.14±1.85)vs.(9.07±2.11)P =0.001，(5.42±1.84)vs.(7.73±1.85)P=0.000 and(6.34±2.06)vs.(8.47±1.91)，P=0.000］There wasnosigni ficantd ifferencein，theaboveindicatorsamongpatientswit，h different degrees of tumor differentiation in the observation group(P＞0.05).Tumor infil? trating dendritic cells density of observation group was obviously lower than that of the control group(8.27±2.11)vs.(9.68±2.38)，P =0.002，percentage of MHC?Ⅱ positive cell significantly lower than the control group(6.67±2.20)vs.(9.79±2.58)，P=0.000，per? centage of CD54 positive cell significantly lower than the control group(7.46±2.31)vs.(9.83±2.54)，P=0.000.Conclusion The density of tumor?infiltrating dendritic cells in the cancer tissue of patients with oral squamous cell carcinoma decreased，and the proportion of MHCⅡ?positive and CD54?positive cells decreased.With the increase of TNM staging and tumor diameter，the expres?sion level of tumor infiltrating dendritic cells is lower. |
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