| 陈宝胜,刘雅刚,张吉水,等.非编码 RNA IncWDR 26对肝癌细胞的抑制作用[J].安徽医药,2020,24(11):2166-2170. |
| 非编码 RNA IncWDR 26对肝癌细胞的抑制作用 |
| Inhibition of non?coding RNA IncWDR 26 on hepatoma cells |
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| DOI：10.3969/j.issn.1009?6469.2020.11.012 |
| 中文关键词: 肝肿瘤/病因学 转染 膜联蛋白质类 IncWDR 26 细胞增殖 细胞周期 非编码 RNA |
| 英文关键词: Liver neoplasms/etiology Transfection Annexins IncWDR 26 Cell proliferation Cell cycle Long non? coding RNAs(lncRNA) |
| 基金项目:沧州市重点研发计划指导项目(183302069) |
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| 中文摘要: |
| 目的探讨非编码 RNA IncWDR 26对肝癌细胞(HCC)的周期、侵袭、迁移以及增殖的影响。方法通过 real?time PCR，比较 SMMC?7721，PLC/PRF/5，Huh7，SK?Hep?1以及 Hep3B肝癌细胞系和正常肝细胞 LO2中 IncWDR 26表达的差异；而后将 In? cWDR 26高表达的慢病毒载体(OE组)和阴性对照载体(Con组)分别转染 SMMC?7721及 SK?Hep?1两株 HCC，通过 CCK?8法检测 IncWDR 26对 HHC增殖的影响；采用 FLUOS分析 HHC细胞周期情况；采用细胞凋亡试剂盒检测 IncWDR 26对 HHC凋亡的影响；采用 transwell法测定其侵袭和迁移能力。结果与正常肝细胞 LO2(1.02±0.27)相比，其余 5株 HCC细胞 IncWDR 26表达水平相对较低(P＜0.05)； OE组中的两株细胞 IncWDR 26表达量显著高于 Con组［SMMC?7721(23.04±4.28)比(1.42±0.38)SK?Hep?1(16.83±3.94)比(1.21±0.29)］(P＜0.05)，OE组中 SMMC?7721及 SK?Hep?1两株细胞培育 1d后的增殖率均显著低于，Con组中的两组细胞(P＜0.05)；两组中 SMMC?7721及 SK?Hep?1两株细胞周期位于 G0/G1，S及 G2/M期的细胞构成比比较差异无统计学意义(P＞0.05)； OE组中的两株细胞中膜联蛋白 V阳性细胞平均率显著高于 Con组中两株细胞［SMMC?7721(20.14± 2.58)%比(10.07±2.02)%，SK?Hep?1(27.45±4.34)%比(13.67±2.38)%］(P＜0.05)； OE组中两株 HCC细胞侵袭检测和迁移检测的平均视野内下室表面细胞数均显著低于 Con组(P＜0.05)。结论非编码 RNA IncWDR 26能有效抑制 HHC增殖的同时，还能进一步降低其侵袭以及迁移能力，从而达到抑制 HCC的作用。 |
| 英文摘要: |
| Objective To investigate the effects of non?coding RNA IncWDR 26 on the cycle，invasion，migration and proliferation of hepatoma cells(HCC).Methods The difference between the expression of IncWDR 26 in SMMC?7721，PLC/PRF/5，Huh7，SK? Hep?1 and Hep3B hepatoma cell lines and normal hepatocytes LO2 was analyzed by real?time PCR；Then，the lentiviral vector(OE group)and the negative control vector(Con group)with high expression of IncWDR 26 were transfected into two HCCs，SMMC? 7721 and SK?Hep?1，respectively.Detection of the effect of IncWDR 26 on HHC proliferation by CCK?8 assay.Analysis of HHC cellcycle using FLUOS.Detection of the effect of IncWDR 26 on HHC apoptosis using an apoptosis kit.The invasion and migration abili?ty was determined by the transwell method.Results Compared with normal liver cell LO2(1.02±0.27)，the expression levels of Inc? WDR 26 in the other 5 HCC cells were relatively low(P＜0.05).The expression of IncWDR 26 in the two cells in the OE group was significantly higher than that in the Con group［SMMC?7721(23.04±4.28)vs.(1.42±0.38)，SK?Hep?1(16.83±3.94)vs.(1.21±0.29)］(P＜0.05).The proliferation rate of SMMC?7721 and SK?Hep?1 cells in the OE group was significantly lower than that in the Con group(P＜0.05).There was no significant difference in cell ratio between SMMC?7721 and SK?Hep?1 in G0/G1，S and G2/ M phases(P＞0.05).The average rate of Annexin V positive cells in the two cells in the OE group was significantly higher thanthat in the Con group［SMMC?7721(20.14±2.58)% vs.(10.07±2.02)%，SK?Hep?1(27.45±4.34)% vs.(13.67±2.38)%］(P＜0.05)； The number of cells in the lower ventricular surface of the two HCC cells in the OE group was significantly lower than that in theCon group(P＜0.05).Conclusion Non?coding RNA IncWDR 26 can effectively inhibit the proliferation of HHC，and further re? duce its invasion and migration，thereby inhibiting HCC. |
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