| 何亚丽,刘宏.生长抑制特异性转录本 5和微小 RNA-142-3p在重症急性胰腺炎并急性呼吸窘迫综合征病人血清中的表达及临床意义[J].安徽医药,2021,25(5):975-979. |
| 生长抑制特异性转录本 5和微小 RNA-142-3p在重症急性胰腺炎并急性呼吸窘迫综合征病人血清中的表达及临床意义 |
| Expression and clinical significance of GAS5 and miR-142-3p in serum of patients with SAP and ARDS |
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| DOI：10.3969/j.issn.1009-6469.2021.05.032 |
| 中文关键词: 胰腺炎，急性坏死性 呼吸窘迫综合征，成人 生长抑制特异性转录本 5(GAS5) 微小 RNA-142-3p 诊断 预后 |
| 英文关键词: Pancreatitis, acute necrotizing Respiratory distress syndrome, adult Growth arrest specific transcript 5(GAS5) miR-142-3p Diagnosis Prognosis |
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| 中文摘要: |
| 目的探讨生长抑制特异性转录本 5(GAS5)和微小 RNA-142-3p(miR-142-3p)在重症急性胰腺炎( SAP)合并急性呼吸窘迫综合征( ARDS)病人血清中的表达及临床意义。方法选取 2017年 2月至 2018年 12月平顶山市第一人民医院 SAP病人 83例，采用随机数字表法抽样 41例并发 ARDS者为 ARDS组， 42例非 ARDS组。另选取同时期 40例健康体检者作为健康对照组。实时荧光定量 PCR(RT-qPCR)检测病人血清 GAS5和 miR-142-3p表达水平， Pearson相关性分析 SAP合并 ARDS病人血清 GAS5与 miR-142-3p表达相关性，受试者工作特征( ROC)曲线分析血清 GAS5和 miR-142-3p对 SAP合并 ARDS的诊断价值， logistic回归分析 SAP合并 ARDS及其预后的影响因素。结果与健康对照组比较，非 ARDS组和 ARDS组病人血清 GAS5表达升高( P<0.05)miR-142-3p表达水平降低( P<0.05)。与非 ARDS组比较， ARDS组病人血清 GAS5表达升高( P<0.05)，miR-1423p表达水平降低，(P<0.05)。 Pearson相关性分析结果显示， SAP合并 ARDS病人血清 GAS5与 miR-142-3p呈负相关(P<0.05)。 GAS5与 miR-142-3p联合检测诊断 SAP合并 ARDS的灵敏度、特异度及曲线下面积( AUC)均高于 GAS5、miR-142-3p单独诊断(P<0.05)。与 SAP合并 ARDS存活病人比较，死亡病人血清 GAS5表达水平升高(P<0.05)，miR-142-3p表达水平降低( P<0.05)。 GAS5和 miR-142-3p是 SAP病人合并 ARDS的显著影响因素( P<0.05)，也是 SAP合并 ARDS病人预后的显著影响因素( P<0.05)。结论 SAP合并 ARDS病人血清中 GAS5表达升高， miR-142-3p表达降低，可能为 SAP合并 ARDS的诊断和预后判断提供了新的生物学指标。 |
| 英文摘要: |
| Objective to investigate the expression and clinical significance of growth suppression-specific transcription 5 (GAS5) and micrna-142-3p (mir-142-3p) in the serum of patients with severe acute pancreatitis (SAP) combined with acute respiratory distress syndrome (ARDS).Methods A total of 41 patients with ARDS were randomly sampled from SAP patients in Pingdingshan First People′s Hospital from February 2017 to December 2018, including the ARDS group and the non-ards group. In addition, 40 healthy physical examinees were selected as the healthy control group. Real-time fluorescence quantitative PCR (rt-qpcr) was used to detect the expression levels of serum GAS5 and mir-142-3p in patients with SAP combined with ARDS. Pearson correlation was used to analyze thecorrelation between the expression levels of serum GAS5 and mir-142-3p in patients with SAP combined with ARDS. ROC curve was used to analyze the diagnostic value of serum GAS5 and mir-142-3p in patients with SAP combined with ARDS.Results Compared with the healthy control group, the expression of serum GAS5 in the non-ARDS group and ARDS group increased (P<0.05), and the expression level of miR-142-3p decreased (P<0.05), and the expression level of mir-142-3p was decreased (P<0.05). Compared with the non-ards group, the expression of serum GAS5 in ARDS group was increased (P<0.05), and the expression level of mir-142-3p was decreased (P<0.05). Pearson correlation analysis results showed that the serum GAS5 of SAP patients with ARDS was negatively correlated with mir-142-3p (P<0.05). The sensitivity, specificity and AUC of both GAS5 and mir-142-3p were higher than that of GAS5 and mir-142-3p alone (P<0.05). Compared with the surviving patients with SAP combined with ARDS, the expression level of serum GAS5in the deceased patients was increased (P<0.05), and the expression level of mir-142-3p was decreased (P<0.05). GAS5 and mir-1423p were significant factors affecting ARDS in SAP patients combined with ARDS (P <0.05) and the prognosis of SAP patients combined with ARDS (P <0.05).Conclusion The expression of GAS5 in the serum of patients with SAP combined with ARDS is increased, and the expression of miR-142-3p is decreased, which may provide new biological indicators for the diagnosis and prognosis of SAP combined with ARDS. |
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