| 唐静月,胡敏敏,卞振华,等.清肺消痤合剂的质量标准研究[J].安徽医药,2021,25(8):1488-1491. |
| 清肺消痤合剂的质量标准研究 |
| Study on the quality standards of Qingfeixiaocuo mixture |
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| DOI：10.3969/j.issn.1009-6469.2021.08.002 |
| 中文关键词: 色谱法，薄层 中草药 清肺消痤合剂 质量标准 黄芩苷 小檗碱 |
| 英文关键词: Chromatography, thin layer Drugs, chinese herbal Qingfeixiaocuo mixture Quality standard Baicalin Berberine |
| 基金项目:无锡市卫生计生委科研项目( MS201839)；全国中药特色技术传承人才培训项目( T20194828003) |
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| 中文摘要: |
| 目的探讨清肺消痤合剂的质量控制标准。方法采用薄层色谱法(TLC)对连翘、黄芩、黄柏进行鉴别；采用高效液相法( HPLC)对黄芩苷和小檗碱进行含量测定。结果在选定的薄层色谱条件下，连翘、黄芩、黄柏的斑点清晰，分离度好，阴性无干扰；黄芩苷、小檗碱分别在 66.40~331.99 μg/mL、18.73~93.64 μg/mL浓度范围内呈良好的线性关系( r为 0.999 7~0.999 8)，平均加样回收率分别为 99.27%、99.65%，RSD分别为 2.80%、2.58 %(n=6)。结论该方法简便准确，重现性好，可作为有效控制清肺消痤合剂质量的方法。 |
| 英文摘要: |
| Objective To explore the quality control standard of Qingfeixiaocuo mixture.Methods The thin layer chromatography(TLC) was used to identify forsythia, Scutellaria baicalensis and golden cypress. The high performance liquid method (HPLC) was usedto determine the contents of baicalin and berberine.Results Forsythia, Scutellaria baicalensis and golden cypress could be detectedand the spots were clear under the selected chromatographic conditions. There was no interference with the negative sample. Baicalinand berberine had good linear relationships in the concentration range of 66.40-331.99, 18.73-93.64 μg/mL, r of 0.999 7-0.999 8. The average recoveries were 99.27 % and 99.65 %, respectively. The RSD were 2.80 % and 2.58 %, respectively (n=6).Conclusion Themethod has the advantages of simple operation, strong accuracy and good reproducibility. It can be used as an effective method to control the quality of Qingfeixiaocuo mixture. |
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