| 彭湃,杨军,邱勇.氯普鲁卡因通过调控长链非编码 RNA TTN?AS1表达对胶质瘤细胞增殖和转移的影响[J].安徽医药,2021,25(11):2140-2143. |
| 氯普鲁卡因通过调控长链非编码 RNA TTN?AS1表达对胶质瘤细胞增殖和转移的影响 |
| Effects of chloroprocaine on the proliferation and metastasis of glioma cells by regulating the expression of lncRNA TTN-AS1 |
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| DOI：10.3969/j.issn.1009-6469.2021.11.005 |
| 中文关键词: 神经胶质瘤 氯普鲁卡因 RNA，反义 lncRNA TTN-AS1 增殖 迁移 侵袭 |
| 英文关键词: Glioma Chloroprocaine RNA, antisense LncRNA TTN-AS1 Proliferation Migration Invasion |
| 基金项目:湖北省自然科学基金( ZRMS2017001731) |
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| 中文摘要: |
| 目的探讨氯普鲁卡因( CP)对胶质瘤细胞增殖和转移的影响及分子机制。方法用浓度为 1 mmol/L、3 mmol/L、9 mmol/L的 CP培养 U251细胞作为 CP低、中、高浓度组；未经任何处理的细胞作为对照组；此外将 U251细胞分为 si-NC组、 siTTN-AS1组、 CP+pcDNA组、 CP+pcDNA-TTN-AS1组。噻唑兰( MTT)检测细胞增殖抑制率； Transwell检测细胞迁移和侵袭；实时荧光定量 PCR(RT-qPCR)检测 lncRNA TTN-AS1表达水平。结果 CP处理胶质瘤 U251细胞后，细胞增殖抑制率升高，细胞迁移侵袭数以及 TTN-AS1表达水平降低( P<0.05)。抑制 TTN-AS1表达可抑制胶质瘤细胞增殖、迁移和侵袭。 TTN-AS1过表达逆转了 CP对 U251细胞的作用。结论 CP通过下调 lncRNA TTN-AS1表达抑制胶质瘤细胞增殖和转移。 |
| 英文摘要: |
| Objective To investigate the effect of chloroprocaine (CP) on the proliferation and metastasis of glioma cells and its molecular mechanism.Methods U251 cells were cultured with chloroprocaine at a concentration of 1 mmol/L, 3 mmol/L, and 9 mmol/Las the low, medium, and high concentration groups of chloroprocaine; cells without any treatment were used as the control group. In addition, U251 cells were divided into si-NC group, si-TTN-AS1 group, CP+pcDNA group, CP+pcDNA-TTN-AS1 group. Thiazole blue(MTT) was used to detect the inhibition rate of cell proliferation; Transwell was used to detect cell migration and invasion; real-time quantitative PCR (RT-qPCR) was used to detect the expression of lncRNA TTN-AS1.Results After chloroprocaine treatment of glioma U251 cells, the cell proliferation inhibition rate was significantly increased, and the number of cell migration and invasion, TTNAS1 expression were reduced (P<0.05). Inhibition of TTN-AS1 expression can inhibit glioma cell proliferation, migration and invasion. TTN-AS1 overexpression reversed the effect of chloroprocaine on U251 cells.Conclusion Chloroprocaine inhibits the proliferation and metastasis of glioma cells by down-regulating the expression of lncRNA TTN-AS1. |
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