| 周文艳,王宇明,黄雁,等.高效液相色谱法测定复方黄黛片中丹酚酸 B的含量[J].安徽医药,2022,26(3):463-465. |
| 高效液相色谱法测定复方黄黛片中丹酚酸 B的含量 |
| Determination of salvianolic acid B in compound realgar and natural indigo tablets by HPLC |
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| DOI：10.3969/j.issn.1009-6469.2022.03.009 |
| 中文关键词: 丹酚酸 B 复方黄黛片 高效液相色谱法 质量控制 |
| 英文关键词: Salvianolic acid B Compound realgar and natural indigo tablets HPLC Quality control |
| 基金项目:安徽省 2018年科技厅重大专项( 18030801127) |
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| 中文摘要: |
| 目的建立高效液相色谱法( HPLC)用于测定复方黄黛片中丹酚酸 B的含量。方法色谱柱为 C18柱( 250 mm×4.6 mm，5 μm)，流动相为乙腈 -0.1%磷酸( 22∶78)，流速为 1.0 mL/min，检测波长为 286 nm，柱温为 35 ℃，进样量为 10 μL。结果丹酚酸 B在 10.076~151.140 mg/L范围内与峰面积呈良好线性关系( r=0.999 9)，平均加样回收率为 95.62%，相对标准差为 |
| 英文摘要: |
| Objective To establish a high-performance liquid chromatography (HPLC) method for the determination of salvianolicacid B in the compound realgar natural indigo tablet.Methods A C18 column (4.6 mm×250 mm, 5 μm) was used, the mobile phase was acetonitrile-0.1% phosphoric acid (22∶78), the flow rate was 1.0 mL/min, the detection wavelength was 286 nm, the column temperature was 35 ℃, and the injection volume was 10 μL.Results Salvianolic acid B showed a good linear relationship with the peak area in the concentration range of 10.076~151.140 mg/L (r=0.999 9), the average recovery was 95.62%, and the relative standard deviation (RSD) was 1.6%.Conclusion The method is simple and accurate, and can be used for the quality control of compound realgar and natural indigo tablets. |
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