| 张海英,郑晨颖.膝骨关节炎关节液和滑膜中微小 RNA-140-5p的表达及临床意义[J].安徽医药,2022,26(6):1179-1182. |
| 膝骨关节炎关节液和滑膜中微小 RNA-140-5p的表达及临床意义 |
| Expression and clinical significance of miR-140-5p in synovial fluid and synovium of knee osteoarthritis |
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| DOI：10.3969/j.issn.1009-6469.2022.06.028 |
| 中文关键词: 骨关节炎，膝 关节液 滑膜 微小 RNA-140-5p |
| 英文关键词: Osteoarthritis, knee Synovial fluid Synovium Microrna-140-5p |
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| 中文摘要: |
| 目的研究膝骨关节炎(KOA)病人关节液和滑膜中微小 RNA(miR)-140-5p的表达及临床意义。方法选取 2018年 5月至 2019年 5月于北京中医药大学东方医院就诊的 51例 KOA病人为观察组，依据凯尔格伦 -劳伦斯( K-L)分级标准将观察组分为 3个亚组: KOA Ⅱ组( 12例)、 KOA Ⅲ组( 24例)、 KOA Ⅳ组( 15例)另外选取 55例同期该院因骨折、截肢等手术治疗的无骨关节炎病人为对照组。采用实时荧光定量逆转录聚合酶链反应( qRT-，PCR)检测并比较各组关节液和滑膜中 miR-140-5p的表达，分析 miR-140-5p与 KOA病情进展的关系；采用受试者工作曲线( ROC)分析 miR-140-5p对 KOA的诊断价值。结果观察组关节液( 0.51±0.16)、滑膜( 0.67±0.21)中 miR-140-5p均比对照组［( 1.08±0.38)、(1.54±0.35)］低( P<0.05)； KOA Ⅱ组、 KOA Ⅲ组、 KOA Ⅳ组相比，关节液［( 0.65±0.17)、(0.51±0.14)、(0.42±0.12)］、滑膜［( 0.86±0.22)、(0.69±0.15)、(0.49±0.13)］中 miR140-5p均呈依次降低趋势，两两相比差异有统计学意义( P<0.05)； Spearman相关性分析结果显示， KOA病人关节液、滑膜中 miR-140-5p与 K-L分级均呈负相关( P<0.05)； ROC结果显示，关节液、滑膜 miR-140-5p诊断 KOA的曲线下面积( AUC)分别为 |
| 英文摘要: |
| Objective To study the expression and clinical significance of microRNA-140-5p (miR-140-5p) in synovial fluid and synovium of patients with knee osteoarthritis (KOA).Methods A total of 51 patients with KOA treated in Dongfang Hospital of BeijingUniversity of Traditional Chinese Medicine from May 2018 to may 2019 were selected as the observation group. According to the Kelgren Lawrence (K-L) classification standard, the observation group was divided into three subgroups: KOA Ⅱ group (12 cases), KOAⅢ group (24 cases), KOA Ⅳ group (15 cases). In addition, 55 patients without osteoarthritis treated by fracture and amputation in thehospital in the same period were selected as the control group. The expression of miR-140-5p in synovial fluid and synovium of each group was detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR), the relationship between miR-140-5p and the progression of KOA was analyzed; and the diagnostic value of miR-140-5p to KOA was analyzed by receiver operating characteristic curve (ROC).Results MiR-140-5p in synovial fluid (0.51±0.16) and synovium (0.67±0.21) of the KOAgroup was lower than that of the control group [(1.08±0.38), (1.54±0.35)] (P < 0.05); miR-140-5p in synovial fluid [(0.65±0.17), (0.51±0.14), 0.42±0.12)] and synovium [(0.86±0.22),(0.69±0.15),(0.49±0.13)] in KOA Ⅱ group, KOA Ⅲ group and KOA Ⅳ group decreasedin turn, the differences between any two groups were statistically significant (P < 0.05); Spearman's results showed that miR-140-5p in synovial fluid and synovium of KOA patients were negatively correlated with K-L grading (P < 0.05); the results of ROC showed that the areas under the curve (AUC) for diagnosing KOA were 0.913 and 0.931 (P < 0.05), respectively, the cutoff values were 0.78 and 1.05,respectively, and the sensitivity, specificity, accuracy, Youden index, positive predictive value, negative predictive value, positive likelihood ratio and negative likelihood ratio were 98.04%, 78.18%, 87.74%, 0.76, 80.65%, 97.73%, 4.49, 0.03; 96.08%, 89.09%, 92.45%,0.85, 89.09%, 96.08%, 8.81, 0.04, respectively.Conclusion The miR-140-5p shows low expression in joint fluid and synovium of patients with KOA, and is negatively correlated with K-L grade, which may become a monitoring index and treatment target for the development of KOA patients. |
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