| Objective To study the effect of Banxia Xiexin decoction on oxidative stress injury in ulcerative colitis mice and explorethe role of nuclear factor erythroid 2 related factor (Nrf2)/heme oxygenase-1 (HO-1) pathway.Methods From January to May 2023,Mice were given 3% dextran sulfate sodium (DSS) for a week to establish the ulcerative colitis model. Sixty male C57BL/6J mice wererandomly divided into control group, model group, mesalazine group (100 mg/kg), Banxia Xiexin decoction high-dose, medium-dose and low-dose groups (32.76, 16.38 and 8.19 g/kg). At the same time, corresponding drugs were given by gavage for 10 consecutive days. Atthe end of the experiment, the body weight and colon length of mice were measured, and the pathological change of colon was observed.Serum reactive oxide species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) were detected by enzyme-linked immunosorbent assay. The mRNA and protein expressions of Nrf2, HO-1 and NADPH quinine oxidoreduc-tase-1 (NQO-1) were detected by RT-PCR and Western blotting.Results The colon length, ROS, MDA, SOD, GSH-px content of micein the control group was (12.53±0.94)cm, (9.39±0.76)U/mL,(4.32±0.37)nmol/L, (124.79±11.27)U/mL, and (1 102.92±87.20)U/mL, re-spectively. The colon length, ROS, MDA, SOD, GSH-px content of mice in the model group was (5.63±0.45)cm, (13.30±1.47)U/mL,(7.65±0.81)nmol/L,(80.31±8.98)U/mL, and (823.00±89.63)U/mL, respectively. Compared with the control group, the body weight and colon length were significantly reduced in the model group,the crypt structure of colonic mucosal tissue was destroyed, goblet cells werereduced,inflammatory cell infiltration was obvious, the contents of ROS and MDA in serum were significantly increased,and the activi-ties of antioxidant enzymes SOD and GSH-px were significantly decreased.The mRNA and protein expressions of Nrf2,HO-1 and NQO-1 were obviously reduced.The colon length,ROS,MDA,SOD,GSH-px content of mice in the high-dose of Banxia Xiexin decoction groupwas (8.97±1.20)cm, (10.49±1.04)U/mL, (5.72±0.57)nmol/L, (112.24±10.85)U/mL, and (1 032.20±117.66)U/mL, respectively. The colonlength,ROS,MDA,SOD,GSH-px content of mice in the medium-dose of Banxia Xiexin decoction group was (8.03±0.69)cm,(11.23±1.11)U/mL,(6.47±0.30)nmol/L,(93.20±11.47)U/mL,and (993.96±96.72)U/mL, respectively.The colon length,ROS,MDA,SOD,GSH-px content of mice in the low-dose of Banxia Xiexin decoction group was (7.53±0.69)cm,(12.33±1.45)U/mL, (7.23±0.65)nmol/L,(84.89±9.07)U/mL,and (891.06±86.61)U/mL, respectively. Compared with the model group, after the intervention of Banxia Xiexin decoction, the bodyweight and colon length of mice were significantly increased,the crypt structure of colon tissue was clear, the infiltration of inflammatorycell was reduced,the contents of ROS and MDA in serum were significantly decreased, and the activities of SOD and GSH-px were re-markably increased.The mRNA and protein expressions of Nrf2,HO-1 and NQO-1 in colon tissues were also significantly increased. Conclusions Banxia Xiexin decoction could improve UC symptoms,regulate the expression level of Nrf2/HO-1 related proteins,then affect the contents of oxidative stress indicators,such as ROS,MDA,SOD and GSH-px,and finally reduce the degree of pathological dam-age of colon.This paper laid a foundation for the development of Banxia Xiexin decoction for the prevention and treatment of UC. |
