| 张静,李静,侯红丽,等.黄芩苷改善呼吸道合胞病毒肺炎幼鼠肺损伤的作用机制[J].安徽医药,2024,28(5):904-908. |
| 黄芩苷改善呼吸道合胞病毒肺炎幼鼠肺损伤的作用机制 |
| Baicalin mediates the differentiation of T lymphocyte subsets through p38MAPK signaling pathway to improve lung injury in young rats with respiratory syncytial virus pneumonia |
| |
| DOI：10.3969/j.issn.1009-6469.2024.05.012 |
| 中文关键词: 黄芩 黄芩苷 呼吸道合胞病毒 肺炎 肺损伤 p38丝裂原活化蛋白激酶 |
| 英文关键词: Scutellaria baicalensis Baicalin Respiratory syncytial virus Pneumonia Lung injury p38 mitogen activated pro-tein kinase |
| 基金项目:河南省医学科技攻关计划(联合共建项目)(2018020664) |
|
| 摘要点击次数: 75 |
| 全文下载次数: 31 |
| 中文摘要: |
| 目的探讨黄芩苷对呼吸道合胞病毒( RSV)肺炎幼鼠肺损伤的改善作用及其可能作用机制。方法 2022年 3月至 2023年 7月自杭州子源实验动物科技有限公司购入 55只 4周龄 SPF级雄性 SD大鼠，选取其中 45只鼻腔接种 50 μLRSV建立 RSV肺炎幼鼠模型，造模成功幼鼠采用随机数字表法分为模型组、黄芩苷低、中和高剂量组，各 10只，另设对照组(10只)给予相应药物进行干预， 1次/天，连续 7d。称取各组幼鼠肺质量，并计算肺指数；肺组织烘干后计算肺组织湿 /干比(W/D)评定肺水，肿； ELISA法检测幼鼠肺泡灌洗液中肿瘤坏死因子 -α(TNF-α)、白细胞介素 -1β(IL-1β)和白细胞介素 -6(IL-6)水平；流式细胞仪检测幼鼠外周血中 T细胞亚群； HE染色观察各组大鼠肺组织病理变化； RT-qPCR和蛋白质印迹法分别检测大鼠肺组织中 p38丝裂原活化蛋白激酶( p38MAPK)及其磷酸化 mRNA和蛋白表达情况。结果黄芩苷低剂量组幼鼠肺指数、肺组织 W/D、TNF-α、IL-1β、IL-6、CD4+CD4+/CD8+p38MAPK mRNA表达和 p-p38MAPK蛋白表达水平［( 1.05±0.06)%、(6.13±0.40)、(392.37±20.82)ng/L、(72.85±42)ng/L3.29±8.59)ng/L、(25.85±1.50)%、(3.98±0.33、0.65±0.08)］，中剂量组［( 0.81±0.06)%、(5.62±0.41)、(308.17±18.87)ng/L、(57.59±4.14)ng/L、(82.36±6.05)ng/L、(23.59±1.30)%、(3.36±0.36、0.54±0.06)］，高剂量组［( 0.72± 0.05)%、(4.03±0.30)、(246.87±16.44)ng/L、(42.15±3.33)ng/L、(56.17±5.13)ng/L、(20.16±0.99)%、(2.17±0.24、0.42±0.05)］均显著低于模型组［( 1.43±0.09)%、(7.82±0.37)、(487.85±25.77)ng/L、(91.24±7.41)ng/L、(132.75±9.70)ng/L、(27.24±1.72)%、(4.75±0.40、0.78±0.11)］(P<0.05)；黄芩苷低剂量组幼鼠体质量、 CD3+水平［( 78.05±1.46)g、(49.66±0.89)%］中剂量组［( 86.04±2.31) g、(51.17±1.04)%］，高剂量组［( 99.34±2.08)g、(54.87±1.25)%］均显著高于模型组［( 72.53±1.56)g(46.25±0.82)%］(P<0.05)。结论黄芩苷能够降低 RSV肺炎幼鼠炎症因子水平，调节 T淋巴细胞亚群比例，减轻肺损伤，可能是通过影响 p38MAPK信号通路发挥作用。 |
| 英文摘要: |
| Objective To investigate the ameliorative effect of Baicalin on lung injury in young rats with respiratory syncytial virus(RSV) pneumonia and its possible mechanism.Methods From March 2022 to July 2023, fifty 4-week-old SPF male SD rats were pur-chased from Hangzhou Ziyuan Laboratory Animal Technology Co., LTD. Forty-five of them were selected for nasal inoculation with 50 μLRSV to establish a RSV pneumonia model. The successful young rats were randomly divided into model group, low, medium andhigh dose of Baicalin groups, with 10 rats in each group, and another control group (10 rats), and the corresponding drugs were given forintervention, once a day for 7 days. The lung mass of young rats in each group was weighed and the lung index was calculated; After thelung tissue was dried, the Wet/Dry ratio (W/D) of lung tissue was calculated to evaluate pulmonary edema;The levels of tumor necrosisfactor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in alveolar lavage fluid of rats was detected by ELISA;T cell subsetsin peripheral blood of young rats were detected by flow cytometry;The pathological changes of lung tissue was observed by HE staining;RT qPCR and Western blot were used to detect the expression of p38 mitogen activated protein kinase(p38MAPK) and its phosphorylat-ed mRNA and protein in rat lung tissue.Results Lung index, lung tissue W/D, TNF-α, IL-1β, IL-6, CD4+, CD4+/CD8+, p38MAPK mRNA expression and p-p38MAPK protein expression levels in baicalin low-dose group[(1.05±0.06)%,(6.13±0.40),(392.37±20.82)ng/L,(72.85±4.82)ng/L,(103.29±8.59)ng/L,(25.85±1.50)%,(3.98±0.33,0.65±0.08)], baicalin medium-dose group[(0.81±0.06)%,(5.62±0.41),(308.17±18.87)ng/L, (57.59±4.14)ng/L, (82.36±6.05)ng/L, (23.59±1.30)% , (3.36±0.36, 0.54±0.06)] and baicalin high-dose group[(0.72±0.05)%,(4.03±0.30),(246.87±16.44)ng/L,(42.15±3.33)ng/L,(56.17±5.13)ng/L,(20.16±0.99)%,(2.17±0.24,0.42±0.05)] were significantly lower than those in the model group[(1.43±0.09)% , (7.82±0.37), (487.85±25.77)ng/L, (91.24±7.41)ng/L, (132.75±9.70)ng/L, (27.24±1.72)%,(4.75±0.40,0.78±0.11)] (P<0.05); The mass of rat and level of CD3+ in baicalin low-dose group[(78.05±1.46)g,(49.66±0.89)%], baicalin medium-dose group[(86.04±2.31)g,(51.17±1.04)%] and baicalin high-dose group[(99.34±2.08)g, (54.87±1.25)% ] were higher than those in the model group[(72.53±1.56)g,(46.25±0.82)%](P<0.05).Conclusion Baicalin can reduce the level of inflammatory fac-tors, regulate the proportion of T lymphocyte subsets and reduce lung injury in young rats with RSV pneumonia, which may play a role by affecting p38MAPK signal pathway. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
