| 赵军,孟西娜,侍佳,等.血清 miR?3614?5p在妊娠期肝内胆汁淤积症诊断中的价值[J].安徽医药,2020,24(3):446-448. |
| 血清 miR?3614?5p在妊娠期肝内胆汁淤积症诊断中的价值 |
| The value of serum miR?3614?5p in the diagnosis of intrahepatic cholestasis of pregnancy |
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| DOI：10.3969/j.issn.1009?6469.2020.03.006 |
| 中文关键词: 妊娠并发症 胆汁淤积，肝内 微 RNAs 荧光免疫测定 诊断 miR?3614?5p |
| 英文关键词: Pregnancy complications Cholestasis,intrahepatic Micro RNAs Fluoroimmunoassay Diagnosis MiR?3614?5p |
| 基金项目:国家自然科学基金( 81671489)；江苏省科技发展重点研发计划( BE2017628) |
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| 中文摘要: |
| 目的探讨血清 miR?3614?5p在妊娠期肝内胆汁淤积症( ICP)诊断中的应用。方法采用临床对照回顾性研究方法。选取南京医科大学附属无锡妇幼保健院 2017年 11至 2018年 10月就诊的 79例 ICP病人，同时按随机数字表法选取同期 79例正常孕妇作为健康对照。采用实时荧光定量 PCR(qPCR)法检测血清 miR?3614?5p水平，并分析 miR?3614?5p与临床资料的关系，进行相关性及受试者工作特征曲线( ROC)分析，评价 miR?3614?5p在 ICP诊断中的临床应用价值。结果 ICP病人血清 miR?3614?5p相对表达量［ΔCT:9.893(8.635，10.166)］明显高于对照组［ΔCT:10.841(10.256，11.987)］，差异有统计学意义( P＜ 0.05)。 ICP组中 miR?3614?5p和 TBA水平呈显著正相关( r＝0.311，P＜0.001)，血清 miR?3614?5p在 ICP组中的 ROC曲线下面积(AUC)、灵敏度和特异度分别为 0.864、79.0%和 83.0%。结论血清 miR?3614?5p可能成为 ICP诊断的新型分子靶标。 |
| 英文摘要: |
| Objective To investigate the application of serum miR?3614?5p in the diagnosis of intrahepatic cholestasis of pregnan? cy(ICP).Methods A clinical controlled retrospective study was used.79 patients with ICP，admitted in The Affiliated Wuxi Ma? temity and Child Health Care Hospital of Nanjing Medical University from November 2017 to October 2018 as 79 healthy pregnantwomen，were selected by random number table.Serum miR?3614?5p levels were measured by qPCR，and the relationship betweenmiR?3614?5p and clinical data were analyzed.Correlation and receiver operating characteristic curve(ROC)analysis were per? formed to evaluate clinical application value of miR?3614?5p in diagnosis of ICP.Results The relative expression of serum miR? 3614?5p in ICP patients［ΔCT:9.893(8.635，10.166)］ was significantly higher than that in the control group［ΔCT:10.841(10.256，11.987)］，the difference was statistically significant(P＜0.05).There was a significant positive correlation between miR? 3614?5p and TBA levels in the ICP group(r＝0.311，P＜0.001).The area under the ROC curve(AUC)，sensitivity，and specificity of serum miR?3614?5p in the ICP group were 0.864、79.0% and 83.0%，respectively.Conclusion Serum miR?3614?5p may be a novel molecular target for ICP diagnosis. |
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