| 王清,曹雨诞,陈佩东.溪黄草中迷迭香酸的含量测定研究[J].安徽医药,2020,24(6):1098-1101. |
| 溪黄草中迷迭香酸的含量测定研究 |
| Content determination of rosmarinic acid in Isodon striatus |
| |
| DOI：10.3969/j.issn.1009?6469.2020.06.009 |
| 中文关键词: 溪黄草/分析 回收率 色谱法，高压液相 含量测定 迷迭香酸 |
| 英文关键词: Isodon striatus /analysis Recovery rate Chromatography,high pressure liquid Content determination Rosmarinic acid |
| 基金项目:江苏省药学会课题(苏食药监法科〔 2014〕337号) |
|
| 摘要点击次数: 1711 |
| 全文下载次数: 581 |
| 中文摘要: |
| 目的测定溪黄草中迷迭香酸的含量。方法采用高效液相色谱 ?质谱法( HPLC?MS)分析，并应用高效液相色谱(HPLC)法测定溪黄草饮片中迷迭香酸含量，应用 Kromasil C18(250 mm×4.6 mm，5 μm)色谱柱，以乙腈、 0.1%磷酸( 25∶75)洗脱并进行方法学考察。结果迷迭香酸在 0.063～1.012 μg/mL，r＝0.999 7范围内呈良好的线性关系，迷迭香酸的平均回收率为 96.40%，相对标准偏差( RSD)为 2.46%。结论该研究所建立的测定方法简便、重现性好，可用于溪黄草的质量控制。 |
| 英文摘要: |
| Objective To determine the content of rosmarinic acid in Isodon striatus.Methods The content of rosmarinic acid was analyzed by high performance liquid chromatography?tandem mass spectrometry(HPLC?MS)and measured by high perfor? mance liquid chromatography(HPLC).A Kromasil C18(250 mm×4.6 mm，5 μm)column was adopted.Acetonitrile and 0.1% phos? phoric acid(25∶75) was used for elution and a methodological investigation was conducted.Results Rosmarinic acid showed good linearity within the range of 0.063?1.012 μg/mL，r＝0.999 7.The average recovery rate was 96.40% and relative standard devi? ation(RSD)was 2.46%.Conclusion The method is convenient and repeatable，which is suitable for the quality control of Isodon striatus. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
