| 张伟,赵卫华,徐云兴,等.茵陈五苓散对实验性梗阻性黄疸大鼠肝损伤的保护作用[J].安徽医药,2020,24(10):1947-1951. |
| 茵陈五苓散对实验性梗阻性黄疸大鼠肝损伤的保护作用 |
| Protection effect of Yinchen?Wuling powder on liver injury in experimental obstructive jaundice rats |
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| DOI：10.3969/j.issn.1009?6469.2020.10.008 |
| 中文关键词: 黄疸，阻塞性 茵陈五苓散 肝损伤 钙蛋白酶 氧化应激 大鼠， Sprague?Dawley |
| 英文关键词: Jaundice,obstructive Yinchen?Wuling powder Liver injury Calpain Oxidative stress Rats,Sprague?Dawley |
| 基金项目:河南省高等学校重点科研项目(15A320010) |
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| 中文摘要: |
| 目的探究茵陈五苓散对实验性梗阻性黄疸大鼠肝损伤的保护作用。方法 2017年 1月至 2019年 5月， 60只 SD大鼠，选取 45只制成实验性梗阻性黄疸大鼠模型，剩余 15只为对照组， 45只模型大鼠按照随机数字表法分为:模型组、低剂量茵陈五苓散组(10 g·kg-1·d-1)和高剂量茵陈五苓散组(20 g·kg-1·d-1)每组各 15只；实验组使用对应浓度茵陈五苓散灌胃，对照组和模型组使用等量生理盐水灌胃，持续 15 d。使用全自动生化分析仪检测各组大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、血清总胆红素(TBIL)和内毒素(ET)水平；使用酶联免疫吸附法(ELISA)检测各组大鼠肝组织匀浆丙二醛(MDA)、超氧化物歧化酶(SOD)含量水平；使用蛋白质印记法检测各组大鼠肝组织凋亡相关蛋白 B淋巴细胞瘤 ?2蛋白(Bcl?2)、 Bcl?2相关 X蛋白(Bax)、半胱氨酸天门冬氨酸特异性蛋白酶(Caspase?3)和钙蛋白酶(Calpain)蛋白表达水平。结果相比对照组，模型组大鼠体质量、肝指数、 ALT、AST、TBIL、ET、MDA、Bax蛋白、 Caspase?3蛋白、 Calpain蛋白水平均显著升高(P＜0.05)SOD、Bcl?2蛋白水平均显著降低(P＜0.05)；相比模型组，低剂量茵陈五苓散组大鼠体质量、肝指数、 ALT、AST、TBIL、ET、MDA、，Bax蛋白、 Caspase?3蛋白、 Calpain蛋白水平分别为［(0.75±0.23)g、(2.53±0.17)、(130.50±12.05)U/L、(125.55±20.95)U/L、(100.10±10.38)μmol/L、(0.52±0.03)EU/mL、(13.50±3.21)U/mg、(1.00±0.15)、(1.20±0.20)、(0.75±0.15)］，高低剂量茵陈五苓散组分别为［(0.69±0.10)g、(2.11±0.13)、(93.15±10.25)U/L、(83.47±12.27)U/L、(72.30±10.25)μmol/L、(0.40±0.03)EU/mL、(9.26±2.20)U/mg、(0.90±0.11)、(1.00±0.19)、(0.45±0.10)］均显著降低(P＜0.05)，且高剂量茵陈五苓散组显著低于低剂量茵陈五苓散组(P＜0.05)；相比模型组，低剂量茵陈五苓散组大鼠 SOD、Bcl?2蛋白水平分别为［(200.47±27.15)U/mg、(0.69±0.23)］，高剂量茵陈五苓散组大鼠分别为［(270.15±32.06)U/mg、(0.75±0.10)］，均显著升高(P＜0.05)且高剂量茵陈五苓散组显著高于低剂量茵陈五苓散组(P＜0.05)。结论茵陈五苓散通过降低脂质过氧化物水平、提高 SOD性、抑制钙蛋白酶的表达同时抑制肝活，细胞的凋亡实现实验性梗阻性黄疸大鼠肝脏的保护作用，且在一定剂量范围内呈剂量依赖性。 |
| 英文摘要: |
| Objective To explore the protection effect of Yinchen?Wuling powder on liver injury in experimental obstructive jaun? dice(EOJ)rats.Methods January 2017 to May 2019，of the 60 SD rats，45 cases were enrolled to be made into EOJ models，and the remaining 15 cases were enrolled as control group.According to random number table method，the 45 model rats were divided into model group，low?dose Yinchen?Wuling powder group(10 g·kg-1·d-1)and high?dose Yinchen?Wuling powder group(20 g·kg-1· d-1)with 15 cases in each group.The experimental groups were gavaged with corresponding concentrations of Yinchen?Wuling pow? der，w，hile control group and model group were gavaged with same amount of normal saline for 15 days.Levels of serum alanine ami?notransferase(ALT)aspartate aminotransferase(AST)，serum total bilirubin(TBIL)and endotoxin(ET)in each group were mea? suredbyfully?automati，c biochemical analyzer.The levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in liver tis? sue homogenate of each group were detected by enzyme linked immunosorbent assay(ELISA).The expression levels of apoptosis?re? lated proteins［B cell lymphoma?2(Bcl?2)，Bcl?2 associated X protein(Bax)，cysteine?containing aspartate?specific protease?3(Caspase?3)］and calpain protein in liver tissue were detected by Western blot.Results Compared with the control group，weight， liver index，levels of ALT，AST，TBIL，ET and MDA，expression levels of Bax protein，Caspase?3 protein and Calpain protein were significantly increased in the model group，while expression levels of SOD and Blc?2 protein were significantly decreased(P＜0.05).Compared with model group，weight，liver index，levels of ALT，AST，TBIL，ET and MDA，expression levels of Bax protein， Caspase?3 protein and Calpain protein were significantly decreased in low?dose Yinchen?Wuling powder group［(0.75±0.23)g，(2.53±0.17)(130.50±12.05)U/L，(125.55±20.95)U/L，(100.10±10.38)μmol/L，(0.52±0.03)EU/mL，(13.50±3.21)U/mg，(1.00±0.15)1.20±0.20)，(0.75±0.15)］ and high?dose Yinchen?Wuling powder group［(0.69±0.10)g，(2.11±0.13)，(93.15±10.25)U/L3.47±12.27)U/L，(72.30±10.25)μmol/L，(0.40±0.03)EU/mL，(9.26±2.20)U/mg，(0.90±0.11)，(1.00±0.19)，(0.45±0.10)］(P＜0.05).And the above indexes in high?dose Yinchen?Wuling powder group were significantly lower than those in low?dose Yinchen?Wuling powder group(P＜0.05).Compared with model group，expression levels of SOD and Blc?2 protein were significantly increased in low?dose Yinchen?Wuling powder group［(200.47±27.15)U/mg，(0.69±0.23)］ and high ?dose Yinchen? Wuling powder group［(270.15±32.06)U/mg，(0.75±0.10)］(P＜0.05).And the above indexes in high?dose Yinchen?Wuling pow? der group were significantly higher than those in low?dose Yinchen?Wuling powder group(P＜0.05).Conclusion Yinchen?Wuling powder can protect liver of EOJ rats by reducing lipid peroxide level，increasing SOD activity，inhibiting calpain expression and he?patocytes apoptosis.The above protective effects are dose?dependent within a certain dose range. |
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