| 汤澎,赵娟,杨光.柚皮素对体外糖尿病视网膜新生血管形成的抑制作用及机制[J].安徽医药,2021,25(11):2165-2169. |
| 柚皮素对体外糖尿病视网膜新生血管形成的抑制作用及机制 |
| Inhibitory effect and mechanism of naringenin on diabetic retinal neovascularization in vitro |
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| DOI：10.3969/j.issn.1009-6469.2021.11.010 |
| 中文关键词: 黄酮类 糖尿病视网膜病变 内皮，血管 柚皮素 视网膜新生血管 自噬 高糖 血管生成 |
| 英文关键词: Flavones Diabetic retinopathy Endothelium, vascular Naringenin Retinal neovascularization Autophagy High glucose Angiogenesis |
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| 中文摘要: |
| 目的观察柚皮素对体外高糖诱导的视网膜血管生成过程的影响及机制。方法将猴视网膜血管内皮细胞( RF/6A)采用随机数字表法分为对照组、高糖组、高糖 +柚皮素组，对照组在常规培养基中培养，高糖组在培养基中加入 30 mmol/L D-葡萄糖，高糖 +柚皮素组在培养基中加入 30 mmol/L D-葡萄糖及 3 mg/L柚皮素。培养 24 h后，分别采用 cck-8、Transwell和 Matrigel实验检测细胞增殖、迁移及管腔形成。采用蛋白质印迹法( Western blotting)检测细胞自噬标志蛋白微管相关蛋白 1轻链 3(LC3)及哺乳动物雷帕霉素靶蛋白(mTOR)的表达。结果高糖组细胞增殖率( 72.08±2.12)%比对照组(99.43±0.67)%减小，高糖＋柚皮素组( 90.54±1.47)%明显高于高糖组；细胞迁移数和管腔形成比较，高糖组明显高于对照组，高糖＋柚皮素组显低于高糖组； Western blotting结果表明高糖组细胞 LC3-Ⅱ的表达明显高于对照组，高糖＋柚皮素组细胞的表达明显低于高糖组；高糖组细胞 p-mTOR的表达明显低于对照组，高糖＋柚皮素组细胞的表达明显高于高糖组。结论柚皮素可通过抑制细胞自噬在体外抑制糖尿病视网膜新生血管形成。 |
| 英文摘要: |
| Objective To observe the effect and mechanism of naringenin on retinal angiogenesis induced by high glucose in vitro. Methods Monkey retinal vascular endothelial cells (RF/6A) were randomly divided into three groups: control group (regular culture),the high glucose group (adding 30 mmol/L D-glucose to the medium), high glucose + naringenin group (adding 30 mmol/L D-glucos and 3 mg/L naringen to the medium). After 24 h of culture, the cck-8, transwell and matrigel assay was used to detect cell proliferation, migration and tube formation, respectively. The expression of microtubule-related protein 1 light chain 3 (LC3) and mammalian target of rapamycin (mTOR) were detected by western blotting.Results The cell proliferation rate of the high-glucose group(72.08±2.12)% was significantly lower than that of the control group(99.43±0.67)%, and this rate of the high-glucose + naringenin group(90.54±1.47)% was significantly higher than that of the high-glucose group. The results of cell migration and tube formation showed that the high-glucosegroup was significantly higher than the control group, and the high glucose + naringenin group was significantly lower than the high-glucose group. Western blotting results showed that the expression of LC3-Ⅱ in the high-glucose group was significantly higher than that in the control group, and that in the high-glucose + naringenin group was significantly lower than that in the high-glucose group. The expression of p-mTOR in the high-glucose group was significantly lower than that in the control group, and that in the high-glucose + naringenin group was significantly higher than that in the high-glucose group.Conclusion Naringenin can inhibit diabetic retinal neovascularization in vitro by inhibiting autophagy. |
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