| 白彦红,陈华,谢影,等.微 RNA-204-5p、蛋白锌指 E盒结合同源盒蛋白 1在新生儿肺炎血清中的表达[J].安徽医药,2024,28(2):362-365. |
| 微 RNA-204-5p、蛋白锌指 E盒结合同源盒蛋白 1在新生儿肺炎血清中的表达 |
| Serum miR-204-5p and ZEB1 levels in neonatal pneumonia and their clinical value in disease assessment and prognosis prediction |
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| DOI：10.3969/j.issn.1009-6469.2024.02.033 |
| 中文关键词: 新生儿肺炎 E盒结合锌指蛋白 1 微 RNA-204-5p 蛋白锌指 E盒结合同源盒蛋白 1 预后 |
| 英文关键词: Eonatal pneumonia Zinc finger E-box-binding homeobox 1 MicroRNA-204-5p Protein zinc finger E-box binding homeobox protein 1 Prognosis |
| 基金项目:保定市科技计划项目( 2141ZF286) |
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| 中文摘要: |
| 目的探讨微 RNA-204-5p(miR-204-5p)、蛋白锌指 E盒结合同源盒蛋白 1(ZEB1)在新生儿肺炎( NP)病儿血清中的表达变化及其在病情评估及预后预测中的临床价值。方法选取 2021年 10月至 2022年 5月张家口市妇幼保健院收治的 NP病儿 80例为 NP组，另选取同期该院出生的健康新生儿 80例为对照组。检测血清 miR-204-5p、ZEB1、肌酸激酶( CK)、肌酸激酶同工酶( CK-MB)及 C反应蛋白( CRP)水平并比较；采用 Pearson法分析 NP病儿血清 miR-204-5p、ZEB1水平及其与临床指标的相关性；应用受试者操作特征( ROC)曲线分析 miR-204-5p、ZEB1及二者联合预测 NP不良预后的价值。结果 NP组血清 miR-204-5p水平( 0.47±0.16比 1.01±0.21)、血氧饱和度［(83.99±6.15)%比( 95.32±6.74)%］显著低于对照组，血清 ZEB1［(4.76±0.71) ng/L比( 2.15±0.36)ng/L］、 CK、CK-MB［( 46.25±12.52)U/L比( 23.22±9.64)U/L］及 CRP水平［( 18.09±4.29)mg/L比( 3.31±0.71)mg/ L］均显著高于对照组( P<0.05)。重症组血清 miR-204-5p水平、血氧饱和度显著低于轻症组，血清 ZEB1、CK、CK-MB及 CRP水平均显著高于轻症组( P<0.05)。血清 miR-204-5p与 CPIS评分、 CK-MB及 CRP水平呈负相关( r=.0.33、.0.30、.0.40，P<0.05)，与血氧饱和度呈正相关( r=0.41，P<0.05)；血清 ZEB1水平与 CPIS评分、 CK-MB及 CRP水平呈正相关( r=0.28、0.37、0.44，P<0.05)，与血氧饱和度呈负相关( r=.0.36，P<0.05)；血清 miR-204-5p与 ZEB1水平呈负相关( r=.0.56，P<0.05)。预后不良组血清 miR-204-5p水平、血氧饱和度低于预后良好组( P<0.05)，血清 ZEB1、CK、CK-MB及 CRP水平均显著高于预后良好组( P<0.05)。 ROC曲线显示， miR-204-5p对 NP不良预后预测的 AUC为 0.89，截断值为 0.47，其灵敏度、特异度分别为 95.12%、72.41%；ZEB1对 NP不良预后预测的 AUC为 0.92，截断值为 5.16，其灵敏度、特异度分别为 87.80%、86.21%；二者联合对预后预测的 AUC为 0.99，明显高于二者单独诊断，其灵敏度、特异度分别为 97.56%、94.83%。结论 miR-204-5p在 NP病儿血清中低表达， ZEB1在 NP病儿血清中高表达，均与 NP病情严重程度有关，二者联合对 NP不良预后具有较高的预测价值。 |
| 英文摘要: |
| Objective To investigate the expression changes in microRNA-204-5p (miR-204-5p) and protein zinc finger E box-bind. ing homeobox protein 1 (ZEB1) in serum of children with neonatal pneumonia (NP) and its clinical value in disease assessment andprognosis prediction. Methods From October 2021 to May 2022, 80 children with NP who were admitted to Maternal and ChildHealth Hospital of Zhangjiakou were regarded as the NP group, another 80 healthy newborns born in our hospital were regarded as thecontrol group. Serum levels of miR-204-5p, ZEB1, CK, CK-MB and CRP were detected and compared; Pearson method was applied to analyze the serum miR-204-5p and ZEB1 levels in children with NP and their correlation with clinical indicators; and ROC curve wasapplied to analyze the value of miR-204-5p, ZEB1 and their combination in predicting the poor prognosis of NP.Results The serum miR-204-5p level(0.47±0.16vs. 1.01±0.21) and blood oxygen saturation[(83.99±6.15)% vs. (95.32±6.74)%] in the NP group were obvi.ously lower than those in the control group, and the serum levels of ZEB1[(4.76±0.71)ng/L vs. (2.15±0.36)ng/L], CK, CK-MB[(46.25± 12.52)U/L vs. (23.22±9.64)U/L] and CRP[(18.09±4.29)mg/L vs. (3.31±0.71)mg/L] were obviously higher than those in the control group (P<0.05). Serum miR-204-5p level and blood oxygen saturation in the severe group were obviously lower than those in the mild group, and serum ZEB1, CK, CK-MB and CRP levels were obviously higher than those in the mild group (P<0.05). Serum miR-204-5p was negatively correlated with CPIS score, CK-MB and CRP levels (r=.0.33,.0.30,.0.40, P<0.05), and positively correlated with blood oxy. gen saturation (r=0.41, P<0.05); serum ZEB1 level was positively correlated with CPIS score, CK-MB and CRP levels (r=0.28, 0.37, 0.44, P<0.05), and negatively correlated with blood oxygen saturation (r=.0.36, P<0.05); serum miR-204-5p was negatively correlated with ZEB1 level (r=.0.56, P<0.05). The serum miR-204-5p level and blood oxygen saturation in the poor prognosis group were lower than those in the good prognosis group (P<0.05), the serum levels of ZEB1, CK, CK-MB and CRP were obviously higher than those in the good prognosis group (P<0.05). The ROC curve showed that the AUC of miR-204-5p for predicting poor prognosis in NP was 0.89, the cut-off value was 0.47, and its sensitivity and specificity were 95.12% and 72.41%, respectively; the AUC of ZEB1 for predictingpoor prognosis in NP was 0.92, the cut-off value was 5.16, and its sensitivity and specificity were 87.80% and 86.21%, respectively; theAUC of the combination of the two for prognosis was 0.99, which was obviously higher than that of the two alone, the sensitivity andspecificity were 97.56% and 94.83%, respectively.Conclusions The expression of miR-204-5p is low and the expression of ZEB1 ishigh in the serum of NP children, and both are related to the severity of NP disease. The combination of the two has a high predictivevalue for poor prognosis of NP. |
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